- Catalog number
- EK0939
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Chemokine (C-C motif) ligand 4, also known as CCL4, is a protein which in mouse is encoded by the CCL4 gene. It is a CC chemokine with specificity for CCR5 receptors. It is a chemoattractant for natural killer cells, monocytes and a variety of other immune cells. CCL4 is a major HIV-suppressive factor produced by CD8+ T cells. Performing-low memory CD8+ T cells that normally synthesize MIP-1-beta. By analysis of somatic cell hybrids and by in situ hybridization, the CCL4 gene was assigned to 17q21-q23. Based on functional analysis of MIP-1beta, one or more additional beta chemokine receptors that transduce MIP-1beta signals must also exist. MIP-1alpha and MIP-1beta have been shown to lack affinity for the erythrocyte surface chemokine receptor/Duffy antigen that binds many alpha as well as beta chemokines (11, 14) .
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 31 27 31 27 29 2 6.8% Sample 2 144 160 147 149 150 6.04 4% Sample 3 453 458 508 520 484 29.6 6.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 31 27 31 27 29 2 6.8% Sample 1 31 27 31 27 29 2 6.8% Sample 2 144 160 147 149 150 6.04 4% Sample 2 144 160 147 149 150 6.04 4% Sample 3 453 458 508 520 484 29.6 6.1% Sample 3 453 458 508 520 484 29.6 6.1%
- Synonyms
- C-C motif chemokine 4; Macrophage inflammatory protein 1-beta; MIP-1-beta; Small-inducible cytokine A4; Ccl4; Mip1b, Scya4
- Gene Name
- CCL4
- Gene ID
- 116637
- UniProt
- P50230
- Reactivity
- Rat
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: E.coli; Immunogen sequence: A24-N92
- Capture Antibody
- Monoclonal antibody from mouse
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Field of Research
- Antiviral Signaling, Chemokines, Immune System Diseases, Immunology, Innate Immunity, Macrophage/Inflammation
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin, EDTA, citrate) .
- Detection Range
- 15.6 pg/mL - 1,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 31 144 453 31 148 419 Standard deviation 1.42 9.21 24.31 1.67 12.13 24.3 CV (%) 4.6% 6.4% 5.5% 5.4% 8.2% 5.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 31 144 453 31 148 419 Standard deviation 1.42 9.21 24.31 1.67 12.13 24.3 CV (%) 4.6% 6.4% 5.5% 5.4% 8.2% 5.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 31 144 453 31 148 419 Mean (pg/ml) 31 144 453 31 148 419 Standard deviation 1.42 9.21 24.31 1.67 12.13 24.3 Standard deviation 1.42 9.21 24.31 1.67 12.13 24.3 CV (%) 4.6% 6.4% 5.5% 5.4% 8.2% 5.8% CV (%) 4.6% 6.4% 5.5% 5.4% 8.2% 5.8%
- Sensitivity
- <10 pg/mL
- Function
- Monokine with inflammatory and chemokinetic properties.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 10234 MW
- Specificity
- Natural and recombinant rat CCL4
- Anticoagulant
- Heparin, EDTA or citrate
- Gene Name Synonym
- C-C motif chemokine 4
- Subcellular Location
- Secreted.
- Protein Name
- C-C motif chemokine 4
- Sample Data
- Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.084 0.14 0.207 0.345 0.588 0.966 1.566 2.02 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.084 0.14 0.207 0.345 0.588 0.966 1.566 2.02 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.084 0.14 0.207 0.345 0.588 0.966 1.566 2.02 O.D. 0.084 0.14 0.207 0.345 0.588 0.966 1.566 2.02
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
