- Catalog number
- EK0941
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Melanoma Inhibiting Activity (MIA), also known as cartilage-derived retinoic acid-sensitive protein (CD-RAP), is an approximately 11-15 kDa protein that is expressed as a noncovalent homodimer. MIA is structurally related to OTOR/Otoraplin and MIA-2 in a small family of secreted proteins with one SH3 domain (1-3) . And the MIA gene was mapped to 19q13.32-q13.33 by fluorescence in situ hybridization. Beside, It is a marker for malignant melanoma. MIA functions as a chemoattractant for mesenchymal stem cells and enhances their BMP-2 and TGF-beta3 induced differentiation into chondrocytes . MIA-deficient mice exhibit delayed chondrocyte differentiation but enhanced chondrocyte proliferation and cartilage repair .
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 29 28 27 29 28 0.82 2.9% Sample 2 172 166 171 170 169 2.27 1.3% Sample 3 485 453 511 458 476 23.22 4.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 29 28 27 29 28 0.82 2.9% Sample 1 29 28 27 29 28 0.82 2.9% Sample 2 172 166 171 170 169 2.27 1.3% Sample 2 172 166 171 170 169 2.27 1.3% Sample 3 485 453 511 458 476 23.22 4.8% Sample 3 485 453 511 458 476 23.22 4.8%
- Synonyms
- Melanoma-derived growth regulatory protein; Melanoma inhibitory activity protein; MIA
- Gene Name
- MIA
- Gene ID
- 8190
- UniProt
- Q16674
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: E.coli; Immunogen sequence: G25-131Q
- Capture Antibody
- Monoclonal antibody from mouse
- Detection Antibody
- Polyclonal antibody from goat
- Tissue Specificity
- All malignant melanoma cell lines tested and infrequently in glioma cell lines.
- Applications
- ELISA
- Field of Research
- Cancer, Cytokines, Growth Factors/Hormones, Immunology, Innate Immunity, Secreted Molecules, Signal Transduction, Tumor Immunology
- Standard
- 1 ng/Tube
- Sample Type
- Cell culture supernatants, serum, plasma (heparin, EDTA) and saliva.
- Detection Range
- 15.6 pg/mL - 1,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 29 172 485 26 165 509 Standard deviation 1.91 13.41 32.98 1.97 15.84 36.92 CV (%) 6.6% 7.8% 6.8% 7.6% 9.6% 6.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 29 172 485 26 165 509 Standard deviation 1.91 13.41 32.98 1.97 15.84 36.92 CV (%) 6.6% 7.8% 6.8% 7.6% 9.6% 6.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 29 172 485 26 165 509 Mean (pg/ml) 29 172 485 26 165 509 Standard deviation 1.91 13.41 32.98 1.97 15.84 36.92 Standard deviation 1.91 13.41 32.98 1.97 15.84 36.92 CV (%) 6.6% 7.8% 6.8% 7.6% 9.6% 6.9% CV (%) 6.6% 7.8% 6.8% 7.6% 9.6% 6.9%
- Sensitivity
- <10 pg/mL
- Function
- Elicits growth inhibition on melanoma cells in vitro as well as some other neuroectodermal tumors, including gliomas.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 14509 MW
- Specificity
- Natural and recombinant human MIA
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Melanoma-derived growth regulatory protein
- Subcellular Location
- Secreted.
- Sequence Similarities
- Belongs to the MIA/OTOR family.
- Protein Name
- Melanoma-derived growth regulatory protein
- Sample Data
- Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.019 0.092 0.162 0.314 0.58 1.017 1.664 2.232 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.019 0.092 0.162 0.314 0.58 1.017 1.664 2.232 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.019 0.092 0.162 0.314 0.58 1.017 1.664 2.232 O.D. 0.019 0.092 0.162 0.314 0.58 1.017 1.664 2.232
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
