- Catalog number
- EK0943
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Myeloperoxidase (MPO) is a mammalian phagocyte hemoprotein thought to primarily mediate host defense reactions. It is abundantly expressed in neutrophils and secreted during their activation. Myeloperoxidase is part of the host defense system of human polymorphonuclear leukocytes, responsible for microbicidal activity against a wide range of organisms. It is located in the nucleus as well as in the cytoplasm. Intranuclear MPO may help to protect DNA against damage resulting from oxygen radicals produced during myeloid cell maturation and function.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 354 346 367 309 344 21.55 6.2% Sample 2 1809 1947 1689 1851 1824 92.61 5% Sample 3 5328 4954 4733 4680 4923 255.01 5.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 354 346 367 309 344 21.55 6.2% Sample 1 354 346 367 309 344 21.55 6.2% Sample 2 1809 1947 1689 1851 1824 92.61 5% Sample 2 1809 1947 1689 1851 1824 92.61 5% Sample 3 5328 4954 4733 4680 4923 255.01 5.1% Sample 3 5328 4954 4733 4680 4923 255.01 5.1%
- Synonyms
- Myeloperoxidase; MPO;1.11.2.2; Myeloperoxidase light chain; Myeloperoxidase heavy chain; Mpo
- Gene Name
- MPO
- Gene ID
- 17523
- UniProt
- P11247
- Reactivity
- Mouse
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: M16-T718
- Capture Antibody
- Polyclonal antibody from goat
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Field of Research
- Cancer, Cancer Metabolism, Cardiovascular, Immunology, Innate Immunity, Macrophage/Inflammation, Metabolism, Pathways and Processes, Redox Metabolism, Vasculature
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 156 pg/mL - 10,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 354 1809 5328 362 1718 5171 Standard deviation 19.11 115.77 266.4 346.457 137.44 346.45 CV (%) 5.4% 6.4% 5% 6.9% 8% 6.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 354 1809 5328 362 1718 5171 Standard deviation 19.11 115.77 266.4 346.457 137.44 346.45 CV (%) 5.4% 6.4% 5% 6.9% 8% 6.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 354 1809 5328 362 1718 5171 Mean (pg/ml) 354 1809 5328 362 1718 5171 Standard deviation 19.11 115.77 266.4 346.457 137.44 346.45 Standard deviation 19.11 115.77 266.4 346.457 137.44 346.45 CV (%) 5.4% 6.4% 5% 6.9% 8% 6.7% CV (%) 5.4% 6.4% 5% 6.9% 8% 6.7%
- Sensitivity
- <10 pg/mL
- Function
- Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity (By similarity) . .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 81182 MW
- Specificity
- Natural and recombinant mouse MPO
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Myeloperoxidase
- Subcellular Location
- Lysosome.
- Protein Name
- Myeloperoxidase
- Sample Data
- Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.038 0.155 0.261 0.449 0.831 1.277 1.825 2.085 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.038 0.155 0.261 0.449 0.831 1.277 1.825 2.085 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.038 0.155 0.261 0.449 0.831 1.277 1.825 2.085 O.D. 0.038 0.155 0.261 0.449 0.831 1.277 1.825 2.085
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
