- Catalog number
- EK0945
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- The endothelins is a family of structurally and pharmacologically distinct peptides, which has been identified and sequenced in humans. Three isoforms of human endothelin have been identified: endothelin-1, -2, and -3. Endothelin-1 is a potent, 21-amino acid vasoconstrictor peptide produced by vascular endothelial cells. Endothelins are 21-amino acid vasoconstricting peptides produced primarily in the endothelium having a key role in vascular homeostasis. Endothelins are implicated in vascular diseases of several organ systems, including the heart, general circulation and brain. Endothelins are proteins that constrict blood vessels and raise blood pressure. They are normally kept in balance by other mechanisms, but when they are over-expressed, they contribute to high blood pressure (hypertension) and heart disease.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 11 13 12 14 12 1.11 9.2% Sample 2 51 49 47 47 48 1.65 3.4% Sample 3 131 117 110 124 120 7.82 6.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 11 13 12 14 12 1.11 9.2% Sample 1 11 13 12 14 12 1.11 9.2% Sample 2 51 49 47 47 48 1.65 3.4% Sample 2 51 49 47 47 48 1.65 3.4% Sample 3 131 117 110 124 120 7.82 6.5% Sample 3 131 117 110 124 120 7.82 6.5%
- Synonyms
- Endothelin-1; Preproendothelin-1; PPET1; Endothelin-1; ET-1; Big endothelin-1; EDN1
- Gene Name
- EDN1
- Gene ID
- 1906, 1907, 1908
- UniProt
- P05305, P20800, P14138
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- A peptide reflecting the target protein in human. Sequence: C53-W73
- Capture Antibody
- Monoclonal antibody from rat
- Detection Antibody
- Monoclonal antibody from mouse
- Tissue Specificity
- Expressed in lung, placental stem villi vessels and in cultured placental vascular smooth muscle cells. .
- Applications
- ELISA
- Field of Research
- Cancer, Cancer Metabolism, Cardiovascular, Growth Factors/Hormones, Metabolism, Metabolism Processes, Pathways and Processes, Response To Hypoxia, Signal Transduction, Vasculature
- Standard
- 1 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 3.9 pg/mL - 250 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 11 51 131 11 51 130 Standard deviation 0.62 2.75 8.64 0.62 2.95 10.92 CV (%) 5.7% 5.4% 6.6% 5.7% 5.8% 8.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 11 51 131 11 51 130 Standard deviation 0.62 2.75 8.64 0.62 2.95 10.92 CV (%) 5.7% 5.4% 6.6% 5.7% 5.8% 8.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 11 51 131 11 51 130 Mean (pg/ml) 11 51 131 11 51 130 Standard deviation 0.62 2.75 8.64 0.62 2.95 10.92 Standard deviation 0.62 2.75 8.64 0.62 2.95 10.92 CV (%) 5.7% 5.4% 6.6% 5.7% 5.8% 8.4% CV (%) 5.7% 5.4% 6.6% 5.7% 5.8% 8.4%
- Sensitivity
- <0.5 pg/mL
- Function
- Endothelins are endothelium-derived vasoconstrictor peptides.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 24425 MW
- Specificity
- Natural and recombinant human Endothelin
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Endothelin 1, endothelin 2, endothelin 3
- Subcellular Location
- Secreted.
- Sequence Similarities
- Belongs to the endothelin/sarafotoxin family.
- Protein Name
- Endothelin-1, Endothelin-2, Endothelin-3
- Sample Data
- Concentration (pg/ml) 0 3.9 7.8 15.6 31.2 62.5 125 250 O.D. 0.022 0.081 0.169 0.336 0.672 1.151 1.597 1.88 Concentration (pg/ml) 0 3.9 7.8 15.6 31.2 62.5 125 250 O.D. 0.022 0.081 0.169 0.336 0.672 1.151 1.597 1.88 Concentration (pg/ml) 0 3.9 7.8 15.6 31.2 62.5 125 250 Concentration (pg/ml) 0 3.9 7.8 15.6 31.2 62.5 125 250 O.D. 0.022 0.081 0.169 0.336 0.672 1.151 1.597 1.88 O.D. 0.022 0.081 0.169 0.336 0.672 1.151 1.597 1.88
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
