- Catalog number
- EK0949
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Kallikrein-14 is a protein that in humans is encoded by the KLK14 gene. Kallikrein-14 belongs to the kallikrein subgroup of serine proteases, which have diverse physiologic functions in many tissues. Kallikrein-14 gene is identified in the kallikrein gene cluster on chromosome 19q13.4. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. Kallikrein-14 may activate or inactivate the proteinase-activated receptors F2R, F2RL1 and F2RL3 and other kallikreins including KLK1, KLK3, KLK5 and KLK11.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 28 29 33 31 30 1.92 6.4% Sample 2 211 184 201 205 200 10.03 5% Sample 3 512 489 430 464 473 30.43 6.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 28 29 33 31 30 1.92 6.4% Sample 1 28 29 33 31 30 1.92 6.4% Sample 2 211 184 201 205 200 10.03 5% Sample 2 211 184 201 205 200 10.03 5% Sample 3 512 489 430 464 473 30.43 6.4% Sample 3 512 489 430 464 473 30.43 6.4%
- Synonyms
- Kallikrein-14; hK14;3.4.21.-; Kallikrein-like protein 6; KLK-L6; KLK14; KLKL6
- Gene Name
- KLK14
- Gene ID
- 9622
- UniProt
- Q9P0G3
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: Q19-M248
- Capture Antibody
- Monoclonal antibody from mouse
- Detection Antibody
- Polyclonal antibody from goat
- Tissue Specificity
- Highly expressed in CNS, bone marrow and fetal liver. Also expressed in breast, thyroid, kidney, colon, pancreas, spleen, prostate, uterus, small intestine, placenta and skeletal muscle. Among 40 tissues tested, the highest expression is detected in skin followed by breast and prostate (at protein level) . Expressed in stratum corneum by sweat ducts and sweat glands and detected in sweat (at protein level) . .
- Applications
- ELISA
- Field of Research
- Cancer, Cell Biology, Cell Type Markers, Cytoskeleton/ECM, ECM Enzymes, Extracellular Matrix, Invasion/Microenvironment, Proteolysis/Ubiquitin, Proteolytic Enzymes, Signal Transduction, Tags & Cell Markers, Tumor Associated
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 15.6 pg/mL - 1,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 28 211 512 29 216 500 Standard deviation 2.1 10.12 29.69 2.72 13.39 37.5 CV (%) 7.5% 4.8% 5.8% 9.4% 6.2% 7.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 28 211 512 29 216 500 Standard deviation 2.1 10.12 29.69 2.72 13.39 37.5 CV (%) 7.5% 4.8% 5.8% 9.4% 6.2% 7.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 28 211 512 29 216 500 Mean (pg/ml) 28 211 512 29 216 500 Standard deviation 2.1 10.12 29.69 2.72 13.39 37.5 Standard deviation 2.1 10.12 29.69 2.72 13.39 37.5 CV (%) 7.5% 4.8% 5.8% 9.4% 6.2% 7.5% CV (%) 7.5% 4.8% 5.8% 9.4% 6.2% 7.5%
- Sensitivity
- <2 pg/mL
- Function
- Serine-type endopeptidase with a dual trypsin-like and chymotrypsin-like substrate specificity. May activate/inactivate the proteinase-activated receptors F2R, F2RL1 and F2RL3 and other kallikreins including KLK1, KLK3, KLK5 and KLK11. May function in seminal clot liquefaction through cleavage of the semenogelin SEMG1 and SEMG2 and activation of KLK3. May function through desmoglein DSG1 cleavage in epidermal desquamation a process by which the most superficial corneocytes are shed from the skin surface. May be involved in several aspects of tumor progression including growth, invasion and angiogenesis. .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 29122 MW
- Specificity
- Natural and recombinant human Kallikrein 14
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Kallikrein-14
- Subcellular Location
- Secreted, extracellular space .
- Sequence Similarities
- Belongs to the peptidase S1 family. Kallikrein subfamily.
- Protein Name
- Kallikrein-14
- Sample Data
- Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.054 0.136 0.216 0.385 0.677 1.185 1.819 2.285 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.054 0.136 0.216 0.385 0.677 1.185 1.819 2.285 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.054 0.136 0.216 0.385 0.677 1.185 1.819 2.285 O.D. 0.054 0.136 0.216 0.385 0.677 1.185 1.819 2.285
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
