- Catalog number
- EK0958
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Thymic stromal lymphopoietin, also called TSLP is a protein belonging to the cytokine family. This gene is mapped to 5q22.1. It encodes a hemopoietic cytokine proposed to signal through a heterodimeric receptor complex composed of the thymic stromal lymphopoietin receptor and the IL-7R alpha chain. It mainly impacts myeloid cells and induces the release of T cell-attracting chemokines from monocytes and enhances the maturation of CD11c (+) dendritic cells. The protein promotes T helper type 2 (TH2) cell responses that are associated with immunity in various inflammatory diseases, including asthma, allergic inflammation and chronic obstructive pulmonary disease. The protein is therefore considered a potential therapeutic target for the treatment of such diseases.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 89 106 95 91 95 6.57 6.9% Sample 2 239 245 235 240 239 3.56 1.4% Sample 3 883 960 834 974 912 57.16 6.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 89 106 95 91 95 6.57 6.9% Sample 1 89 106 95 91 95 6.57 6.9% Sample 2 239 245 235 240 239 3.56 1.4% Sample 2 239 245 235 240 239 3.56 1.4% Sample 3 883 960 834 974 912 57.16 6.2% Sample 3 883 960 834 974 912 57.16 6.2%
- Synonyms
- Thymic stromal lymphopoietin; TSLP
- Gene Name
- TSLP
- Gene ID
- 85480
- UniProt
- Q969D9
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: E.coli; Immunogen sequence: Y29-Q159
- Capture Antibody
- Monoclonal antibody from mouse
- Detection Antibody
- Polyclonal antibody from goat
- Tissue Specificity
- Isoform 1 is expressed in a number of tissues including heart, liver and prostate. Isoform 2 is the predominant form in keratinocytes of oral mucosa, skin and in salivary glands. It is secreted into saliva. .
- Applications
- ELISA
- Field of Research
- Cytokines, Immunology, Innate Immunity
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 31.2 pg/mL - 2,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 89 239 883 94 243 964 Standard deviation 4.36 17.92 45.21 5.82 19.92 65.55 CV (%) 4.9% 7.5% 5.2% 6.2% 8.2% 6.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 89 239 883 94 243 964 Standard deviation 4.36 17.92 45.21 5.82 19.92 65.55 CV (%) 4.9% 7.5% 5.2% 6.2% 8.2% 6.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 89 239 883 94 243 964 Mean (pg/ml) 89 239 883 94 243 964 Standard deviation 4.36 17.92 45.21 5.82 19.92 65.55 Standard deviation 4.36 17.92 45.21 5.82 19.92 65.55 CV (%) 4.9% 7.5% 5.2% 6.2% 8.2% 6.8% CV (%) 4.9% 7.5% 5.2% 6.2% 8.2% 6.8%
- Sensitivity
- <10 pg/mL
- Function
- Isoform 1: Cytokine that induces the release of T-cell- attracting chemokines from monocytes and, in particular, enhances the maturation of CD11c (+) dendritic cells. Can induce allergic inflammation by ly activating mast cells. .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 18141 MW
- Specificity
- Natural and recombinant human TSLP
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Thymic stromal lymphopoietin
- Subcellular Location
- Secreted .
- Protein Name
- Thymic stromal lymphopoietin
- Sample Data
- Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.055 0.1 0.146 0.222 0.356 0.588 1.016 1.822 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.055 0.1 0.146 0.222 0.356 0.588 1.016 1.822 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.055 0.1 0.146 0.222 0.356 0.588 1.016 1.822 O.D. 0.055 0.1 0.146 0.222 0.356 0.588 1.016 1.822
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
