- Catalog number
- EK0959
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Programmed cell death protein 1, also called PD-1 or PDCD1 is a protein that in humans is encoded by the PDCD1 gene. This gene encodes a cell surface membrane protein of the immunoglobulin superfamily. By fluorescence in situ hybridization, the PDCD1 gene is mapped to 2q37.3. This protein is expressed in pro-B-cells and is thought to play a role in their differentiation. In mice, expression of this gene is induced in the thymus when anti-CD3 antibodies are injected and large numbers of thymocytes undergo apoptosis. Mice deficient for this gene bred on a BALB/c background developed dilated cardiomyopathy and died from congestive heart failure. These studies suggest that this gene product may also be important in T cell function and contribute to the prevention of autoimmune diseases.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 17 25 30 19 23 0.41 1.8% Sample 2 88 94 101 79 91 2.35 2.6% Sample 3 267 294 315 302 295 18.26 6.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 17 25 30 19 23 0.41 1.8% Sample 1 17 25 30 19 23 0.41 1.8% Sample 2 88 94 101 79 91 2.35 2.6% Sample 2 88 94 101 79 91 2.35 2.6% Sample 3 267 294 315 302 295 18.26 6.2% Sample 3 267 294 315 302 295 18.26 6.2%
- Synonyms
- Programmed cell death protein 1; Protein PD-1; hPD-1; CD279; PDCD1; PD1
- Gene Name
- PDCD1
- Gene ID
- 5133
- UniProt
- Q15116
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: L25-Q167
- Capture Antibody
- Polyclonal antibody from goat
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Field of Research
- Apoptosis, Associated Proteins, Cell Biology, Developmental Biology, Intracellular, Neurogenesis, Neurology Process, Neuroscience, Organogenesis, Protein Phosphorylation, Ser/Thr Kinases, Signal Transduction
- Standard
- 10 ng/Tube
- Sample Type
- Serum and plasma (heparin) .
- Detection Range
- 31.2 pg/mL - 2,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 17 88 267 22 96 314 Standard deviation 0.71 4.49 13.88 1.12 4.99 19.78 CV (%) 4.2% 5.1% 5.2% 5.1% 5.2% 6.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 17 88 267 22 96 314 Standard deviation 0.71 4.49 13.88 1.12 4.99 19.78 CV (%) 4.2% 5.1% 5.2% 5.1% 5.2% 6.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 17 88 267 22 96 314 Mean (pg/ml) 17 88 267 22 96 314 Standard deviation 0.71 4.49 13.88 1.12 4.99 19.78 Standard deviation 0.71 4.49 13.88 1.12 4.99 19.78 CV (%) 4.2% 5.1% 5.2% 5.1% 5.2% 6.3% CV (%) 4.2% 5.1% 5.2% 5.1% 5.2% 6.3%
- Sensitivity
- <10 pg/mL
- Function
- Inhibitory cell surface receptor involved in the regulation of T-cell function during immunity and tolerance. Upon ligand binding, inhibits T-cell effector functions in an antigen- specific manner. Possible cell death inducer, in association with other factors. .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 31647 MW
- Specificity
- Natural and recombinant human PD-1
- Anticoagulant
- Heparin
- Gene Name Synonym
- Programmed cell death protein 1
- Subcellular Location
- Membrane; Single-pass type I membrane protein.
- Protein Name
- Programmed cell death protein 1
- Sample Data
- Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.019 0.161 0.244 0.384 0.652 1.02 1.74 2.611 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.019 0.161 0.244 0.384 0.652 1.02 1.74 2.611 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.019 0.161 0.244 0.384 0.652 1.02 1.74 2.611 O.D. 0.019 0.161 0.244 0.384 0.652 1.02 1.74 2.611
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
