Human Cathepsin S / CTSS ELISA Kit PicoKine®

About this product

Human Cathepsin S / CTSS ELISA Kit PicoKine® (96 Tests) . Quantitate Human CTSS in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK0961

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

Cathepsin S, also known as CTSS, is a protein that in humans is encoded by the CTSS gene. A member of the peptidase C1 family, CTSS gene is a lysosomal cysteine proteinase that may participate in the degradation of antigenic proteins to peptides for presentation on MHC class II molecules. By fluorescence in situ hybridization (FISH), this gene is mapped to 1q21.3. The encoded protein can function as an elastase over a broad pH range in alveolar macrophages. The bond-specificity of this proteinase is in part similar to the specificities of cathepsin L and cathepsin N.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 177 184 173 178 178 3.93 2.2% Sample 2 404 438 372 391 401 24.07 6% Sample 3 1975 2028 1929 1866 1949 59.59 3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 177 184 173 178 178 3.93 2.2% Sample 1 177 184 173 178 178 3.93 2.2% Sample 2 404 438 372 391 401 24.07 6% Sample 2 404 438 372 391 401 24.07 6% Sample 3 1975 2028 1929 1866 1949 59.59 3% Sample 3 1975 2028 1929 1866 1949 59.59 3%

Synonyms

Cathepsin S;3.4.22.27; CTSS

Gene Name

CTSS

Gene ID

1520

UniProt

P25774

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q17-I331 & S109-I331

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Field of Research

Adaptive Immunity, Cell Biology, Cell Type Markers, Immunology, Non-CD, Proteolysis/Ubiquitin, Proteolytic Enzymes

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

62.5 pg/mL - 4,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 177 404 1975 188 415 1910 Standard deviation 10.08 19.79 126.4 11.65 26.14 154.71 CV (%) 5.7% 4.9% 6.4% 6.2% 6.3% 8.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 177 404 1975 188 415 1910 Standard deviation 10.08 19.79 126.4 11.65 26.14 154.71 CV (%) 5.7% 4.9% 6.4% 6.2% 6.3% 8.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 177 404 1975 188 415 1910 Mean (pg/ml) 177 404 1975 188 415 1910 Standard deviation 10.08 19.79 126.4 11.65 26.14 154.71 Standard deviation 10.08 19.79 126.4 11.65 26.14 154.71 CV (%) 5.7% 4.9% 6.4% 6.2% 6.3% 8.1% CV (%) 5.7% 4.9% 6.4% 6.2% 6.3% 8.1%

Sensitivity

<10 pg/mL

Function

Thiol protease. Key protease responsible for the removal of the invariant chain from MHC class II molecules. The bond- specificity of this proteinase is in part similar to the specificities of cathepsin L and cathepsin N.

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

37496 MW

Specificity

Natural and recombinant human Cathepsin S

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Cathepsin S

Subcellular Location

Lysosome.

Protein Name

Cathepsin S

Sample Data

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.081 0.181 0.249 0.456 0.675 1.084 1.564 2.03 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.081 0.181 0.249 0.456 0.675 1.084 1.564 2.03 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.081 0.181 0.249 0.456 0.675 1.084 1.564 2.03 O.D. 0.081 0.181 0.249 0.456 0.675 1.084 1.564 2.03

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.