- Catalog number
- EK0963
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- MHC class I polypeptide-related sequence B, also called MICB is a protein that in humans is encoded by the MICB gene. The MICB gene is mapped to 6p21.33. This gene encodes a heavily glycosylated protein which is a ligand for the NKG2D type II receptor. Binding of the ligand activates the cytolytic response of natural killer (NK) cells, CD8 alphabeta T cells, and gammadelta T cells which express the receptor. This protein is stress-induced and is similar to MHC class I molecules; however, it does not associate with beta-2-microglobulin or bind peptides. It seems to have no role in antigen presentation. This gene acts as a stress-induced self-antigen that is recognized by gamma delta T cells. It is the ligand for the KLRK1/NKG2D receptor and binds to KLRK1 leads to cell lysis.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 174 167 176 157 168 7.43 4.4% Sample 2 694 670 590 703 664 44.53 6.7% Sample 3 1403 1209 1282 1179 1268 86.34 6.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 174 167 176 157 168 7.43 4.4% Sample 1 174 167 176 157 168 7.43 4.4% Sample 2 694 670 590 703 664 44.53 6.7% Sample 2 694 670 590 703 664 44.53 6.7% Sample 3 1403 1209 1282 1179 1268 86.34 6.8% Sample 3 1403 1209 1282 1179 1268 86.34 6.8%
- Synonyms
- MHC class I polypeptide-related sequence B; MIC-B; MICB ; PERB11.2
- Gene Name
- MICB
- Gene ID
- 4277
- UniProt
- Q29980
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: A23-G298
- Capture Antibody
- Monoclonal antibody from mouse
- Detection Antibody
- Polyclonal antibody from goat
- Tissue Specificity
- Widely expressed with the exception of the central nervous system where it is absent. Expressed in many, but not all, epithelial tumors of lung, breast, kidney, ovary, prostate and colon. In hepatocellular carcinomas, expressed in tumor cells but not in surrounding non-cancerous tissue. .
- Applications
- ELISA
- Field of Research
- Adaptive Immunity, Class I, Immunology
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 62.5 pg/mL - 4,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 174 694 1403 180 758 1442 Standard deviation 9.91 50.66 78.56 12.24 56.85 82.19 CV (%) 5.7% 7.3% 5.6% 6.8% 7.5% 5.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 174 694 1403 180 758 1442 Standard deviation 9.91 50.66 78.56 12.24 56.85 82.19 CV (%) 5.7% 7.3% 5.6% 6.8% 7.5% 5.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 174 694 1403 180 758 1442 Mean (pg/ml) 174 694 1403 180 758 1442 Standard deviation 9.91 50.66 78.56 12.24 56.85 82.19 Standard deviation 9.91 50.66 78.56 12.24 56.85 82.19 CV (%) 5.7% 7.3% 5.6% 6.8% 7.5% 5.7% CV (%) 5.7% 7.3% 5.6% 6.8% 7.5% 5.7%
- Sensitivity
- <10 pg/mL
- Function
- Seems to have no role in antigen presentation. Acts as a stress-induced self-antigen that is recognized by gamma delta T cells. Ligand for the KLRK1/NKG2D receptor. Binding to KLRK1 leads to cell lysis. .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 42646 MW
- Specificity
- Natural and recombinant human MICB
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- MHC class I polypeptide-related sequence B
- Subcellular Location
- Cell membrane ; Single-pass type I membrane protein . Binding to human cytomegalovirus glycoprotein UL16 causes sequestration in the endoplasmic reticulum. .
- Sequence Similarities
- Belongs to the MHC class I family. MIC subfamily.
- Protein Name
- MHC class I polypeptide-related sequence B
- Sample Data
- Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.015 0.193 0.352 0.556 0.885 1.411 1.83 2.023 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.015 0.193 0.352 0.556 0.885 1.411 1.83 2.023 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.015 0.193 0.352 0.556 0.885 1.411 1.83 2.023 O.D. 0.015 0.193 0.352 0.556 0.885 1.411 1.83 2.023
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
