- Catalog number
- EK0973
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Granulin, also known as Proepithelin (PEPI) is a protein that in humans is encoded by the GRN gene. This gene is mapped to 17q21.31. Granulins are a family of secreted, glycosylated peptides that are cleaved from a single precursor protein with 7.5 repeats of a highly conserved 12-cysteine granulin/epithelin motif. The 88 kDa precursor protein, progranulin, is also called proepithelin and PC cell-derived growth factor. Cleavage of the signal peptide produces mature granulin which can be further cleaved into a variety of active, 6 kDa peptides. These smaller cleavage products are named granulin A, granulin B, granulin C, etc. Epithelins 1 and 2 are synonymous with granulins A and B, respectively. Both the peptides and intact granulin protein regulate cell growth. However, different members of the granulin protein family may act as inhibitors, stimulators, or have dual actions on cell growth. Granulin family members are important in normal development, wound healing, and tumorigenesis.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 507 529 566 377 495 28.05 5.7% Sample 2 1173 1005 874 1096 1037 69.89 6.7% Sample 3 9591 8789 9119 5790 8322 566.75 6.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 507 529 566 377 495 28.05 5.7% Sample 1 507 529 566 377 495 28.05 5.7% Sample 2 1173 1005 874 1096 1037 69.89 6.7% Sample 2 1173 1005 874 1096 1037 69.89 6.7% Sample 3 9591 8789 9119 5790 8322 566.75 6.8% Sample 3 9591 8789 9119 5790 8322 566.75 6.8%
- Synonyms
- Granulins; Proepithelin; PEPI; Acrogranin; Glycoprotein of 88 Kda; GP88; Glycoprotein 88; Progranulin; Paragranulin; Granulin-1; Granulin G; Granulin-2; Granulin F; Granulin-3; Granulin B; Granulin-4; Granulin A; Granulin-5; Granulin C; Granulin-6; Granulin D; Granulin-7; Granulin E; GRN
- Gene Name
- GRN
- Gene ID
- 2896
- UniProt
- P28799
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: T18-L593
- Capture Antibody
- Polyclonal antibody from goat
- Detection Antibody
- Polyclonal antibody from goat
- Tissue Specificity
- In myelogenous leukemic cell lines of promonocytic, promyelocytic, and proerythroid lineage, in fibroblasts, and very strongly in epithelial cell lines. Present in inflammatory cells and bone marrow. Highest levels in kidney.
- Applications
- ELISA
- Field of Research
- Cancer, Immunology, Neurodegenerative Disease, Neurology Process, Neuroscience, Secreted Molecules, Signal Transduction
- Standard
- 100 ng/Tube
- Sample Type
- Cell culture supernatants, serum, plasma (heparin, EDTA), saliva, urine and human milk.
- Detection Range
- 312 pg/mL - 20,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 507 1173 9591 518 1169 9230 Standard deviation 36.35 71.9 321.3 16.37 88.96 699.63 CV (%) 7.2% 6.1% 3.4% 3.2% 7.6% 7.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 507 1173 9591 518 1169 9230 Standard deviation 36.35 71.9 321.3 16.37 88.96 699.63 CV (%) 7.2% 6.1% 3.4% 3.2% 7.6% 7.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 507 1173 9591 518 1169 9230 Mean (pg/ml) 507 1173 9591 518 1169 9230 Standard deviation 36.35 71.9 321.3 16.37 88.96 699.63 Standard deviation 36.35 71.9 321.3 16.37 88.96 699.63 CV (%) 7.2% 6.1% 3.4% 3.2% 7.6% 7.6% CV (%) 7.2% 6.1% 3.4% 3.2% 7.6% 7.6%
- Sensitivity
- <150 pg/mL
- Function
- Granulins have possible cytokine-like activity. They may play a role in inflammation, wound repair, and tissue remodeling.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 63544 MW
- Specificity
- Natural and recombinant human Progranulin
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Granulins
- Subcellular Location
- Secreted.
- Sequence Similarities
- Belongs to the granulin family.
- Protein Name
- Granulins
- Sample Data
- Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.039 0.08 0.116 0.221 0.394 0.701 1.15 1.992 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.039 0.08 0.116 0.221 0.394 0.701 1.15 1.992 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.039 0.08 0.116 0.221 0.394 0.701 1.15 1.992 O.D. 0.039 0.08 0.116 0.221 0.394 0.701 1.15 1.992
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
