- Catalog number
- EK0979
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Interleukin-31, also known as IL-31 is a protein that in humans is encoded by the IL31 gene. This gene is mapped to 12q24.31. IL31, which is made principally by activated Th2-type T cells, interacts with a heterodimeric receptor consisting of IL31RA and OSMR that is constitutively expressed on epithelial cells and keratinocytes. IL31 may be involved in the promotion of allergic skin disorders and in regulating other allergic diseases, such as asthma. IL31 may function in skin immunity.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 134 122 129 116 125 6.83 5.4% Sample 2 804 765 827 741 784 33.38 4.2% Sample 3 1907 1624 1623 1803 1739 121.45 6.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 134 122 129 116 125 6.83 5.4% Sample 1 134 122 129 116 125 6.83 5.4% Sample 2 804 765 827 741 784 33.38 4.2% Sample 2 804 765 827 741 784 33.38 4.2% Sample 3 1907 1624 1623 1803 1739 121.45 6.9% Sample 3 1907 1624 1623 1803 1739 121.45 6.9%
- Synonyms
- Interleukin-31; IL-31; IL31
- Gene Name
- IL31
- Gene ID
- 386653
- UniProt
- Q6EBC2
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: E.coli; Immunogen sequence: S24-T164
- Capture Antibody
- Monoclonal antibody from mouse
- Detection Antibody
- Polyclonal antibody from goat
- Tissue Specificity
- Detected at low levels in testis, bone marrow, skeletal muscle, kidney, colon, thymus, small intestine and trachea. .
- Applications
- ELISA
- Field of Research
- Cytokines, Immunology, Innate Immunity, Interleukins
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 62.5 pg/mL - 4,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 134 804 1907 140 740 1811 Standard deviation 8.04 44.22 122.04 10.5 52.54 130.39 CV (%) 6% 5.5% 6.4% 7.5% 7.1% 7.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 134 804 1907 140 740 1811 Standard deviation 8.04 44.22 122.04 10.5 52.54 130.39 CV (%) 6% 5.5% 6.4% 7.5% 7.1% 7.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 134 804 1907 140 740 1811 Mean (pg/ml) 134 804 1907 140 740 1811 Standard deviation 8.04 44.22 122.04 10.5 52.54 130.39 Standard deviation 8.04 44.22 122.04 10.5 52.54 130.39 CV (%) 6% 5.5% 6.4% 7.5% 7.1% 7.2% CV (%) 6% 5.5% 6.4% 7.5% 7.1% 7.2%
- Sensitivity
- <10 pg/mL
- Function
- Activates STAT3 and possibly STAT1 and STAT5 through the IL31 heterodimeric receptor composed of IL31RA and OSMR. IL31 may function in skin immunity. .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 18205 MW
- Specificity
- Natural and recombinant human IL-31
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Interleukin-31
- Subcellular Location
- Secreted .
- Protein Name
- Interleukin-31
- Sample Data
- Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.055 0.137 0.21 0.357 0.676 1.174 1.733 2.333 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.055 0.137 0.21 0.357 0.676 1.174 1.733 2.333 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.055 0.137 0.21 0.357 0.676 1.174 1.733 2.333 O.D. 0.055 0.137 0.21 0.357 0.676 1.174 1.733 2.333
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
