- Catalog number
- EK0985
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Periostin, also known as OSF2, is a protein that in humans is encoded by the POSTN gene. The International Radiation Hybrid Mapping Consortium mapped the POSTN gene to chromosome 13. Periostin functions as a ligand for alpha-V/beta-3 and alpha-V/beta-5 integrins to support adhesion and migration of epithelial cells. It is found that periostin was overexpressed by the majority of human primary breast cancers examined. After myocardial infarction, periostin-induced cardiomyocyte cell cycle reentry and mitosis were associated with improved ventricular remodeling and myocardial function, reduced fibrosis and infarct size, and increase angiogenesis.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 188 183 213 194 194 11.36 5.8% Sample 2 1707 1857 1800 1793 1789 53.58 2.9% Sample 3 4824 4787 4733 4555 4724 103.2 2.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 188 183 213 194 194 11.36 5.8% Sample 1 188 183 213 194 194 11.36 5.8% Sample 2 1707 1857 1800 1793 1789 53.58 2.9% Sample 2 1707 1857 1800 1793 1789 53.58 2.9% Sample 3 4824 4787 4733 4555 4724 103.2 2.1% Sample 3 4824 4787 4733 4555 4724 103.2 2.1%
- Synonyms
- Periostin; PN; Osteoblast-specific factor 2; OSF-2; POSTN; OSF2
- Gene Name
- POSTN
- Gene ID
- 10631
- UniProt
- Q15063
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: N22-Q836
- Capture Antibody
- Monoclonal antibody from mouse
- Detection Antibody
- Monoclonal antibody from mouse
- Tissue Specificity
- Widely expressed with highest levels in aorta, stomach, lower gastrointestinal tract, placenta, uterus, thyroid tissue and breast. Up-regulated in epithelial ovarian tumors. Not expressed in normal ovaries. Also highly expressed at the tumor periphery of lung carcinoma tissue but not within the tumor. Overexpressed in breast cancers. .
- Applications
- ELISA
- Field of Research
- Atherosclerosis, Cancer, Cardiogenesis, Cardiovascular, Cell Adhesion Molecules, Cell Type Markers, Cytoskeleton/ECM, Extracellular Matrix, Invasion/Microenvironment, Signal Transduction, Structures, Tags & Cell Markers, Vascular Inflammation
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 156 pg/mL - 10,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 188 1707 4824 180 1664 4867 Standard deviation 8.64 124.61 192.96 11.34 136.44 199.54 CV (%) 4.6% 7.3% 4% 8.2% 8.2% 4.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 188 1707 4824 180 1664 4867 Standard deviation 8.64 124.61 192.96 11.34 136.44 199.54 CV (%) 4.6% 7.3% 4% 8.2% 8.2% 4.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 188 1707 4824 180 1664 4867 Mean (pg/ml) 188 1707 4824 180 1664 4867 Standard deviation 8.64 124.61 192.96 11.34 136.44 199.54 Standard deviation 8.64 124.61 192.96 11.34 136.44 199.54 CV (%) 4.6% 7.3% 4% 8.2% 8.2% 4.1% CV (%) 4.6% 7.3% 4% 8.2% 8.2% 4.1%
- Sensitivity
- <10 pg/mL
- Function
- Enhances incorporation of BMP1 in the fibronectin matrix of connective tissues, and subsequent proteolytic activation of lysyl oxidase LOX (By similarity) . Induces cell attachment and spreading and plays a role in cell adhesion. May play a role in extracellular matrix mineralization. .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 93314 MW
- Specificity
- Natural and recombinant human Periostin
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Periostin
- Subcellular Location
- Golgi apparatus . Secreted, extracellular space, extracellular matrix . Colocalizes with BMP1 in the Golgi. .
- Sequence Similarities
- Contains 1 EMI domain.
- Protein Name
- Periostin
- Sample Data
- Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.016 0.078 0.128 0.25 0.442 0.853 1.434 2.097 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.016 0.078 0.128 0.25 0.442 0.853 1.434 2.097 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.016 0.078 0.128 0.25 0.442 0.853 1.434 2.097 O.D. 0.016 0.078 0.128 0.25 0.442 0.853 1.434 2.097
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
