- Catalog number
- EK0993
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- OX-2 membrane glycoprotein, also known as CD200 is a human protein encoded by the CD200 gene. This gene is mapped to 3q13.2. The protein encoded by this gene is a type-1 membrane glycoprotein, which contains two immunoglobulin domains, and thus belongs to the immunoglobulin superfamily. Studies of the related genes in mouse and rat suggest that this gene may regulate myeloid cell activity and delivers an inhibitory signal for the macrophage lineage in diverse tissues. It is suggested that CD200-CD200R signaling plays a central role in macrophage fusion and osteoclast formation.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 80 78 90 88 84 5.09 6% Sample 2 246 237 227 247 239 8.07 3.3% Sample 3 894 868 899 880 885 12.15 1.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 80 78 90 88 84 5.09 6% Sample 1 80 78 90 88 84 5.09 6% Sample 2 246 237 227 247 239 8.07 3.3% Sample 2 246 237 227 247 239 8.07 3.3% Sample 3 894 868 899 880 885 12.15 1.3% Sample 3 894 868 899 880 885 12.15 1.3%
- Synonyms
- OX-2 membrane glycoprotein; CD200; CD200; MOX1, MOX2; My033
- Gene Name
- CD200
- Gene ID
- 4345
- UniProt
- P41217
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: Q31-G232
- Capture Antibody
- Polyclonal antibody from goat
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Field of Research
- Adaptive Immunity, Atherosclerosis, Cardiovascular, Immunology, T Cells, Vascular Inflammation
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 31.2 pg/mL - 2,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 80 246 894 73 239 980 Standard deviation 3.76 12.54 68.83 4.16 14.81 77.42 CV (%) 4.7% 7.9% 7.7% 5.7% 6.2% 7.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 80 246 894 73 239 980 Standard deviation 3.76 12.54 68.83 4.16 14.81 77.42 CV (%) 4.7% 7.9% 7.7% 5.7% 6.2% 7.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 80 246 894 73 239 980 Mean (pg/ml) 80 246 894 73 239 980 Standard deviation 3.76 12.54 68.83 4.16 14.81 77.42 Standard deviation 3.76 12.54 68.83 4.16 14.81 77.42 CV (%) 4.7% 7.9% 7.7% 5.7% 6.2% 7.9% CV (%) 4.7% 7.9% 7.7% 5.7% 6.2% 7.9%
- Sensitivity
- <10 pg/mL
- Function
- Costimulates T-cell proliferation. May regulate myeloid cell activity in a variety of tissues.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 31264 MW
- Specificity
- Natural and recombinant human CD200
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- OX-2 membrane glycoprotein
- Subcellular Location
- Cell membrane; Single-pass type I membrane protein.
- Sequence Similarities
- Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
- Protein Name
- OX-2 membrane glycoprotein
- Sample Data
- Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.045 0.133 0.194 0.31 0.523 0.866 1.485 2.336 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.045 0.133 0.194 0.31 0.523 0.866 1.485 2.336 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.045 0.133 0.194 0.31 0.523 0.866 1.485 2.336 O.D. 0.045 0.133 0.194 0.31 0.523 0.866 1.485 2.336
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
