- Catalog number
- EK1110
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- FAS Ligand (FASL) is a 40 kDa type II membrane protein belonging to the tumor necrosis factor family, which induces apoptosis by binding to its receptor, Fas. The human FasL gene consists of approximately 8.0 kb and is split into four exons. This gene consists of 281 amino acids with a calculated M (r) of 31,759 and was mapped on chromosome 1q23. It has an identity of 76.9% at the amino acid sequence level with mouse FasL. The FAS and FASL system plays a key role in regulating apoptotic cell death and corruption of this signalling pathway has been shown to participate in immune escape and tumorigenesis. FAS and FASL triggered apoptosis pathway plays an important role in human carcinogenesis. This system may also play a role in modulating the genetic susceptibility of mouse strains to develop T-cell lymphoblastic lymphomas.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 78 71 76 77 75 2.69 3.5% Sample 2 266 267 298 284 278 13.21 4.7% Sample 3 931 902 822 837 873 45 5.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 78 71 76 77 75 2.69 3.5% Sample 1 78 71 76 77 75 2.69 3.5% Sample 2 266 267 298 284 278 13.21 4.7% Sample 2 266 267 298 284 278 13.21 4.7% Sample 3 931 902 822 837 873 45 5.1% Sample 3 931 902 822 837 873 45 5.1%
- Synonyms
- Tumor necrosis factor ligand superfamily member 6; CD95 ligand; CD95-L; Fas antigen ligand; Fas ligand; FasL; CD178; Tumor necrosis factor ligand superfamily member 6, membrane form; Tumor necrosis factor ligand superfamily member 6, soluble form; Receptor-binding FasL ectodomain; Soluble Fas ligand; sFasL; ADAM10-processed FasL form; APL; FasL intracellular domain; FasL ICD; SPPL2A-processed FasL form; SPA; Faslg; Apt1Lg1, Cd95l, Fasl, Tnfsf6
- Gene Name
- FASLG
- Gene ID
- 25385
- UniProt
- P36940
- Reactivity
- Rat
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: Q127-L278
- Capture Antibody
- Monoclonal antibody from mouse
- Detection Antibody
- Polyclonal antibody from goat
- Tissue Specificity
- Expressed in activated splenocytes and thymocytes. Moderate or weak expression found in small intestines, kidney and lung. .
- Applications
- ELISA
- Field of Research
- Apoptosis, Caspases, Cell Biology, Cytokines, Hematopoietic Progenitors, Immunology, Innate Immunity, Intracellular, Lymphoid, Receptors, Stem Cells, T Lymphocytic Lineage, TNF Superfamily
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin, EDTA, citrate) .
- Detection Range
- 31.2 pg/mL - 2,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 78 266 931 83 263 1019 Standard deviation 3.97 11.17 40.03 4.31 12.88 61.14 CV (%) 6%% 4.2% 4.3% 5.2% 4.9% 6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 78 266 931 83 263 1019 Standard deviation 3.97 11.17 40.03 4.31 12.88 61.14 CV (%) 6%% 4.2% 4.3% 5.2% 4.9% 6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 78 266 931 83 263 1019 Mean (pg/ml) 78 266 931 83 263 1019 Standard deviation 3.97 11.17 40.03 4.31 12.88 61.14 Standard deviation 3.97 11.17 40.03 4.31 12.88 61.14 CV (%) 6%% 4.2% 4.3% 5.2% 4.9% 6% CV (%) 6%% 4.2% 4.3% 5.2% 4.9% 6%
- Sensitivity
- <10 pg/mL
- Function
- Cytokine that binds to TNFRSF6/FAS, a receptor that transduces the apoptotic signal into cells (PubMed:7505205) . Involved in cytotoxic T-cell-mediated apoptosis, natural killer cell-mediated apoptosis and in T-cell development (PubMed:7505205) . Initiates fratricidal/suicidal activation- induced cell death (AICD) in antigen-activated T-cells contributing to the termination of immune responses (By similarity) . TNFRSF6/FAS-mediated apoptosis has also a role in the induction of peripheral tolerance (By similarity) . Binds to TNFRSF6B/DcR3, a decoy receptor that blocks apoptosis (By similarity) . .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 31140 MW
- Specificity
- Natural and recombinant rat FAS-L
- Anticoagulant
- Heparin, EDTA or citrate
- Gene Name Synonym
- Tumor necrosis factor ligand superfamily member 6
- Subcellular Location
- Cell membrane ; Single-pass type II membrane protein . Cytoplasmic vesicle lumen . Lysosome lumen . Colocalizes with the SPPL2A protease at the cell membrane. Is internalized into multivesicular bodies of secretory lysosomes after phosphorylation by FGR and monoubiquitination. .
- Protein Name
- Tumor necrosis factor ligand superfamily member 6
- Sample Data
- Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.033 0.065 0.101 0.173 0.329 0.645 1.251 2.289 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.033 0.065 0.101 0.173 0.329 0.645 1.251 2.289 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.033 0.065 0.101 0.173 0.329 0.645 1.251 2.289 O.D. 0.033 0.065 0.101 0.173 0.329 0.645 1.251 2.289
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
