- Catalog number
- EK1128
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Chemokine (C-C motif) ligand 12 (CCL12) is a small cytokine belonging to the CC chemokine family that has been described in mice. It is also known as monocyte chemotactic protein 5 (MCP-5) and, due to its similarity with the human chemokine MCP-1, sometimes it is called MCP-1-related chemokine. CCL12 specifically attracts eosinophils, monocytes and lymphocytes. This chemokine is found predominately in lymph nodes and thymus under normal conditions, and its expression can be hugely induced in macrophages. It is thought to coordinate cell movements during early allergic reactions, and immune response to pathogens. The gene for CCL12 is found in a cluster of CC chemokines on mouse chromosome 11.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 30 20 18 26 24 1.06 4.5% Sample 2 67 119 123 72 95 3.22 3.4% Sample 3 333 405 405 275 355 27.58 7.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 30 20 18 26 24 1.06 4.5% Sample 1 30 20 18 26 24 1.06 4.5% Sample 2 67 119 123 72 95 3.22 3.4% Sample 2 67 119 123 72 95 3.22 3.4% Sample 3 333 405 405 275 355 27.58 7.8% Sample 3 333 405 405 275 355 27.58 7.8%
- Synonyms
- C-C motif chemokine 12; MCP-1-related chemokine; Monocyte chemoattractant protein 5; Monocyte chemotactic protein 5; MCP-5; Small-inducible cytokine A12; Ccl12; Mcp5, Scya12
- Gene Name
- Ccl12
- Gene ID
- 20293
- UniProt
- Q62401
- Reactivity
- Mouse
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: E.coli; Immunogen sequence: G23-G104
- Capture Antibody
- Monoclonal antibody from rat
- Detection Antibody
- Polyclonal antibody from goat
- Tissue Specificity
- Predominantly expressed in the lymph nodes and thymus. Also found in the salivary glands containing lymph nodes, breast, heart, lung, brain, small intestine, kidney and colon.
- Applications
- ELISA
- Field of Research
- Chemokines, Immunology, Innate Immunity
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 15.6 pg/mL -1000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 30 67 333 30 71 324 Standard deviation 0.70 3.08 18.78 0.66 5.59 15.33 CV (%) 2.3% 4.6% 5.6% 2.2% 7.9% 4.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 30 67 333 30 71 324 Standard deviation 0.70 3.08 18.78 0.66 5.59 15.33 CV (%) 2.3% 4.6% 5.6% 2.2% 7.9% 4.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 30 67 333 30 71 324 Mean (pg/ml) 30 67 333 30 71 324 Standard deviation 0.70 3.08 18.78 0.66 5.59 15.33 Standard deviation 0.70 3.08 18.78 0.66 5.59 15.33 CV (%) 2.3% 4.6% 5.6% 2.2% 7.9% 4.7% CV (%) 2.3% 4.6% 5.6% 2.2% 7.9% 4.7%
- Sensitivity
- <10 pg/mL
- Function
- Chemotactic factor that attracts eosinophils, monocytes, and lymphocytes but not neutrophils. Potent monocyte active chemokine that signals through CCR2. Involved in allergic inflammation and the host response to pathogens and may play a pivotal role during early stages of allergic lung inflammation.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 11659 MW
- Specificity
- Natural and recombinant mouse CCL12
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- C-C motif chemokine 12
- Subcellular Location
- Secreted.
- Protein Name
- C-C motif chemokine 12
- Sample Data
- Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.042 0.106 0.158 0.253 0.46 0.883 1.53 2.088 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.042 0.106 0.158 0.253 0.46 0.883 1.53 2.088 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.042 0.106 0.158 0.253 0.46 0.883 1.53 2.088 O.D. 0.042 0.106 0.158 0.253 0.46 0.883 1.53 2.088
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
