- Catalog number
- EK1129
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- TLR1 is a member of the Toll-like receptor family (TLR) of pattern recognition receptors of the innate immune system. TIR1 gene was mapped to 4p14 by fluorescence in situ hybridization. TLR1 recognizes pathogen-associated molecular pattern with a specificity for gram-positive bacteria. TLR1 has also been designated as CD281 (cluster of differentiation 281) . TLR1 interacts with TLR2 to recognize the lipid configuration of the native mycobacterial lipoprotein as well as several triacylated lipopeptides.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 204 208 203 192 201 5.93 2.9% Sample 2 1142 1045 1032 1050 1067 43.65 4% Sample 3 6099 6066 5527 6006 5924 231.9 3.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 204 208 203 192 201 5.93 2.9% Sample 1 204 208 203 192 201 5.93 2.9% Sample 2 1142 1045 1032 1050 1067 43.65 4% Sample 2 1142 1045 1032 1050 1067 43.65 4% Sample 3 6099 6066 5527 6006 5924 231.9 3.9% Sample 3 6099 6066 5527 6006 5924 231.9 3.9%
- Synonyms
- Toll-like receptor 1; Toll/interleukin-1 receptor-like protein; TIL; CD281; TLR1; KIAA0012
- Gene Name
- TLR1
- Gene ID
- 7096
- UniProt
- Q15399
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: S22-N578
- Capture Antibody
- Monoclonal antibody from mouse
- Detection Antibody
- Polyclonal antibody from goat
- Tissue Specificity
- Ubiquitous. Highly expressed in spleen, ovary, peripheral blood leukocytes, thymus and small intestine.
- Applications
- ELISA
- Field of Research
- Adapters, Immunoglobulins, Immunology, Innate Immunity, Macrophage/Inflammation, NFKB Pathway, Nuclear Signaling, Receptors, Signal Transduction, Signaling Pathway, TLR Signaling, Transmembrane
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 156 pg/mL - 10,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 204 1142 6099 218 1081 6218 Standard deviation 11.22 78.79 378.13 16.2%1 77.83 472.56 CV (%) 5.5% 6.9% 6.2% 7.3% 7.2% 7.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 204 1142 6099 218 1081 6218 Standard deviation 11.22 78.79 378.13 16.2%1 77.83 472.56 CV (%) 5.5% 6.9% 6.2% 7.3% 7.2% 7.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 204 1142 6099 218 1081 6218 Mean (pg/ml) 204 1142 6099 218 1081 6218 Standard deviation 11.22 78.79 378.13 16.2%1 77.83 472.56 Standard deviation 11.22 78.79 378.13 16.2%1 77.83 472.56 CV (%) 5.5% 6.9% 6.2% 7.3% 7.2% 7.6% CV (%) 5.5% 6.9% 6.2% 7.3% 7.2% 7.6%
- Sensitivity
- <10 pg/mL
- Function
- Participates in the innate immune response to microbial agents. Specifically recognizes diacylated and triacylated lipopeptides. Cooperates with TLR2 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (By similarity) . .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 90291 MW
- Specificity
- Natural and recombinant human TLR1
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Toll-like receptor 1
- Subcellular Location
- Cell membrane ; Single-pass type I membrane protein . Cytoplasmic vesicle, phagosome membrane ; Single-pass type I membrane protein .
- Sequence Similarities
- Belongs to the Toll-like receptor family.
- Protein Name
- Toll-like receptor 1
- Sample Data
- Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.017 0.088 0.151 0.258 0.458 0.817 1.395 2.004 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.017 0.088 0.151 0.258 0.458 0.817 1.395 2.004 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.017 0.088 0.151 0.258 0.458 0.817 1.395 2.004 O.D. 0.017 0.088 0.151 0.258 0.458 0.817 1.395 2.004
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
