- Catalog number
- EK1130
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Toll-like receptor 2 also known as TLR-2 is a protein that in humans is encoded by the TLR2 gene. TLR2 has also been designated as CD282 (cluster of differentiation 282) . TLR-2 plays a role in the immune system. TLR-2 is a membrane protein, a receptor, which is expressed on the surface of certain cells and recognizes foreign substances and passes on appropriate signals to the cells of the immune system. TLR2 expresses as 4- and 4.4-kb mRNAs in heart, brain, and muscle.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 304 323 310 307 311 7.24 2.3% Sample 2 1448 1379 1487 1331 1411 60.35 4.2% Sample 3 4661 5150 4522 4797 4782 233.39 4.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 304 323 310 307 311 7.24 2.3% Sample 1 304 323 310 307 311 7.24 2.3% Sample 2 1448 1379 1487 1331 1411 60.35 4.2% Sample 2 1448 1379 1487 1331 1411 60.35 4.2% Sample 3 4661 5150 4522 4797 4782 233.39 4.8% Sample 3 4661 5150 4522 4797 4782 233.39 4.8%
- Synonyms
- Toll-like receptor 2; CD282; Tlr2
- Gene Name
- TLR2
- Gene ID
- 24088
- UniProt
- Q9QUN7
- Reactivity
- Mouse
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: Q25-L590
- Capture Antibody
- Monoclonal antibody from rat
- Detection Antibody
- Polyclonal antibody from goat
- Tissue Specificity
- Detected in a macrophage cell line, smooth muscle, lung, spleen, thymus, brain and adipose tissue.
- Applications
- ELISA
- Field of Research
- Adapters, Atherosclerosis, Cardiovascular, Defensin, Human Protein, Immunoglobulins, Immunology, Innate Immunity, Macrophage/Inflammation, Microbiology, Protein, Receptors, Signal Transduction, TLR Signaling, Transmembrane, Vascular Inflammation
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 156 pg/mL - 10,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 304 1448 4661 301 1423 4925 Standard deviation 21.58 65.76 195.76 26.48 91.07 280.72 CV (%) 7.1% 4.5% 4.2% 8.8% 6.4% 5.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 304 1448 4661 301 1423 4925 Standard deviation 21.58 65.76 195.76 26.48 91.07 280.72 CV (%) 7.1% 4.5% 4.2% 8.8% 6.4% 5.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 304 1448 4661 301 1423 4925 Mean (pg/ml) 304 1448 4661 301 1423 4925 Standard deviation 21.58 65.76 195.76 26.48 91.07 280.72 Standard deviation 21.58 65.76 195.76 26.48 91.07 280.72 CV (%) 7.1% 4.5% 4.2% 8.8% 6.4% 5.7% CV (%) 7.1% 4.5% 4.2% 8.8% 6.4% 5.7%
- Sensitivity
- <10 pg/mL
- Function
- Cooperates with LY96 to mediate the innate immune response to bacterial lipoproteins and other microbial cell wall components. Cooperates with TLR1 or TLR6 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. May also promote apoptosis in response to lipoproteins (By similarity) . .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 89449 MW
- Specificity
- Natural and recombinant mouse TLR2
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Toll-like receptor 2
- Subcellular Location
- Cell membrane; Single-pass type I membrane protein. Cytoplasmic vesicle, phagosome membrane; Single-pass type I membrane protein.
- Sequence Similarities
- Belongs to the Toll-like receptor family.
- Protein Name
- Toll-like receptor 2
- Sample Data
- Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.015 0.079 0.127 0.223 0.389 0.734 1.254 1.891 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.015 0.079 0.127 0.223 0.389 0.734 1.254 1.891 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.015 0.079 0.127 0.223 0.389 0.734 1.254 1.891 O.D. 0.015 0.079 0.127 0.223 0.389 0.734 1.254 1.891
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
