- Catalog number
- EK1136
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Interleukin 17F, also called IL17F is involved in the regulation of normal versus aberrant T-cell responses. This gene is mapped to 6p12.2. The protein encoded by this gene is a cytokine that shares sequence similarity with IL17. This cytokine is expressed by activated T cells, and has been shown to stimulate the production of several other cytokines, including IL6, IL8, and CSF2/GM_CSF. This cytokine is also found to inhibit the angiogenesis of endothelial cells and induce endothelial cells to produce IL2, TGFB1/TGFB, and monocyte chemoattractant protein-1. It is suggested that targeting IL17 and IL17F or antagonizing IL17R might mitigate neutrophil-mediated inflammation in CF.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 57 56 56 53 55 1.5 2.7% Sample 2 188 195 219 193 198 11.96 6% Sample 3 966 948 1023 893 957 46.4 4.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 57 56 56 53 55 1.5 2.7% Sample 1 57 56 56 53 55 1.5 2.7% Sample 2 188 195 219 193 198 11.96 6% Sample 2 188 195 219 193 198 11.96 6% Sample 3 966 948 1023 893 957 46.4 4.8% Sample 3 966 948 1023 893 957 46.4 4.8%
- Synonyms
- Interleukin-17F; IL-17F; Il17f
- Gene Name
- IL17F
- Gene ID
- 301291
- UniProt
- Q5BJ95
- Reactivity
- Rat
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: E.coli; Immunogen sequence: A19-A153
- Capture Antibody
- Monoclonal antibody from mouse
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Field of Research
- Angiogenesis, Cancer, Cardiovascular, Cytokines, Immunology, Innate Immunity, Interleukins, Invasion/Microenvironment
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 31.2 pg/mL - 2,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 57 188 966 55 186 917 Standard deviation 3.3 10.15 41.53 3.96 12.46 52.26 CV (%) 5.8% 5.4% 4.3% 7.2% 6.7% 5.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 57 188 966 55 186 917 Standard deviation 3.3 10.15 41.53 3.96 12.46 52.26 CV (%) 5.8% 5.4% 4.3% 7.2% 6.7% 5.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 57 188 966 55 186 917 Mean (pg/ml) 57 188 966 55 186 917 Standard deviation 3.3 10.15 41.53 3.96 12.46 52.26 Standard deviation 3.3 10.15 41.53 3.96 12.46 52.26 CV (%) 5.8% 5.4% 4.3% 7.2% 6.7% 5.7% CV (%) 5.8% 5.4% 4.3% 7.2% 6.7% 5.7%
- Sensitivity
- <10 pg/mL
- Function
- Stimulates the production of other cytokines such as IL- 6, IL-8 and granulocyte colony-stimulating factor, and can regulate cartilage matrix turnover. Stimulates PBMC and T-cell proliferation. Inhibits angiogenesis (By similarity) . .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 17712 MW
- Specificity
- Natural and recombinant rat IL-17F
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Interleukin-17F
- Subcellular Location
- Secreted .
- Sequence Similarities
- Belongs to the IL-17 family.
- Protein Name
- Interleukin-17F
- Sample Data
- Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.012 0.126 0.206 0.328 0.554 0.923 1.516 2.371 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.012 0.126 0.206 0.328 0.554 0.923 1.516 2.371 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.012 0.126 0.206 0.328 0.554 0.923 1.516 2.371 O.D. 0.012 0.126 0.206 0.328 0.554 0.923 1.516 2.371
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
