- Catalog number
- EK1137
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Chemokine (C-C motif) ligand 8, also known as monocyte chemoattractant protein 2 (MCP-2), is a protein that in humans is encoded by the CCL8 gene. CCL8 is a small cytokine belonging to the CC chemokine family. The CCL8 protein is produced as a precursor containing 109 amino acids, which is cleaved to produce mature CCL8 containing 75 amino acids. The gene for CCL8 is encoded by 3 exons and is located within a large cluster of CC chemokines on chromosome 17q11.2 in humans. Monocyte chemotactic protein 2 (MCP-2) is a CC chemokine that utilizes multiple cellular receptors to attract and activate human leukocytes.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 37 36 34 32 34 1.92 5.6% Sample 2 190 188 192 174 186 7.07 3.8% Sample 3 475 541 471 504 497 28.03 5.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 37 36 34 32 34 1.92 5.6% Sample 1 37 36 34 32 34 1.92 5.6% Sample 2 190 188 192 174 186 7.07 3.8% Sample 2 190 188 192 174 186 7.07 3.8% Sample 3 475 541 471 504 497 28.03 5.6% Sample 3 475 541 471 504 497 28.03 5.6%
- Synonyms
- C-C motif chemokine 8; Monocyte chemoattractant protein 2; Monocyte chemotactic protein 2; MCP-2; Small-inducible cytokine A8; Ccl8; Mcp2, Scya8
- Gene Name
- CCL8
- Gene ID
- 20307
- UniProt
- Q9Z121
- Reactivity
- Mouse
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: E.coli; Immunogen sequence: E20-P97
- Capture Antibody
- Monoclonal antibody from rat
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Field of Research
- Chemokines, Immunology, Innate Immunity, Macrophage/Inflammation
- Standard
- 1 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 15.6 pg/mL - 1,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 37 190 475 33 180 452 Standard deviation 2.66 9.12 28.5 2.73 9 27.12 CV (%) 7.2% 4.8% 6% 8.3% 5% 6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 37 190 475 33 180 452 Standard deviation 2.66 9.12 28.5 2.73 9 27.12 CV (%) 7.2% 4.8% 6% 8.3% 5% 6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 37 190 475 33 180 452 Mean (pg/ml) 37 190 475 33 180 452 Standard deviation 2.66 9.12 28.5 2.73 9 27.12 Standard deviation 2.66 9.12 28.5 2.73 9 27.12 CV (%) 7.2% 4.8% 6% 8.3% 5% 6% CV (%) 7.2% 4.8% 6% 8.3% 5% 6%
- Sensitivity
- <10 pg/mL
- Function
- Chemotactic factor that attracts monocytes. This protein can bind heparin (By similarity) . .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 11017 MW
- Specificity
- Natural and recombinant mouse CCL8
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- C-C motif chemokine 8
- Subcellular Location
- Secreted .
- Protein Name
- C-C motif chemokine 8
- Sample Data
- Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.055 0.137 0.182 0.295 0.496 0.897 1.563 2.225 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.055 0.137 0.182 0.295 0.496 0.897 1.563 2.225 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.055 0.137 0.182 0.295 0.496 0.897 1.563 2.225 O.D. 0.055 0.137 0.182 0.295 0.496 0.897 1.563 2.225
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
