- Catalog number
- EK1141
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Serum paraoxonase/arylesterase 1 (PON1) also known as aromatic esterase 1 or serum aryldialkylphosphatase 1 is an enzyme that in humans is encoded by the PON1 gene. The PON gene was mapped to chromosome 7q21-q22, and the mouse Pon1 gene was mapped to the proximal end of chromosome 6. PON1 is responsible for hydrolysing organophosphate pesticides and nerve gasses. Polymorphisms in the PON1 gene significantly affect the catalytic ability of the enzyme. PON1 (paraoxonase 1) is also a major anti-atherosclerotic component of high-density lipoprotein (HDL) .
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 49 52 45 46 48 2.73 5.6% Sample 2 203 223 226 223 218 9.17 4.2% Sample 3 917 923 806 907 888 47.82 5.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 49 52 45 46 48 2.73 5.6% Sample 1 49 52 45 46 48 2.73 5.6% Sample 2 203 223 226 223 218 9.17 4.2% Sample 2 203 223 226 223 218 9.17 4.2% Sample 3 917 923 806 907 888 47.82 5.3% Sample 3 917 923 806 907 888 47.82 5.3%
- Synonyms
- Serum paraoxonase/arylesterase 1; PON 1;3.1.1.2;3.1.1.81;3.1.8.1; Aromatic esterase 1; A-esterase 1; K-45; Serum aryldialkylphosphatase 1; PON1; PON
- Gene Name
- PON1
- Gene ID
- 5444
- UniProt
- P27169
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: A2-L355
- Capture Antibody
- Monoclonal antibody from mouse
- Detection Antibody
- Polyclonal antibody from goat
- Tissue Specificity
- Plasma, associated with HDL (at protein level) . Expressed in liver, but not in heart, brain, placenta, lung, skeletal muscle, kidney or pancreas. .
- Applications
- ELISA
- Field of Research
- Atherosclerosis, Cancer, Cardiovascular, Cell Biology, Diabetes, Diabetes-associated, Drug Metabolism, Heart Disease, Lipid and Lipoprotein Metabolism, Lipid Metabolism, Lipids/Lipoproteins, Metabolic Signaling Pathways, Metabolism, Neurodegenerative Disease, Neurology Process, Neuroscience, Oxidative Stress, Pathways and Processes, Redox Metabolism, Signal Transduction
- Standard
- 2 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin) .
- Detection Range
- 31.2 pg/mL - 2,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 49 203 917 47 199 834 Standard deviation 2.15 13.19 42.18 2.16 15.52 40.86 CV (%) 4.4% 6.5% 4.6% 4.6% 7.8% 4.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 49 203 917 47 199 834 Standard deviation 2.15 13.19 42.18 2.16 15.52 40.86 CV (%) 4.4% 6.5% 4.6% 4.6% 7.8% 4.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 49 203 917 47 199 834 Mean (pg/ml) 49 203 917 47 199 834 Standard deviation 2.15 13.19 42.18 2.16 15.52 40.86 Standard deviation 2.15 13.19 42.18 2.16 15.52 40.86 CV (%) 4.4% 6.5% 4.6% 4.6% 7.8% 4.9% CV (%) 4.4% 6.5% 4.6% 4.6% 7.8% 4.9%
- Sensitivity
- <10 pg/mL
- Function
- Hydrolyzes the toxic metabolites of a variety of organophosphorus insecticides. Capable of hydrolyzing a broad spectrum of organophosphate substrates and lactones, and a number of aromatic carboxylic acid esters. Mediates an enzymatic protection of low density lipoproteins against oxidative modification and the consequent series of events leading to atheroma formation. .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 39731 MW
- Specificity
- Natural and recombinant human PON1
- Anticoagulant
- Heparin
- Gene Name Synonym
- Serum paraoxonase/arylesterase 1
- Subcellular Location
- Secreted, extracellular space.
- Sequence Similarities
- Belongs to the paraoxonase family.
- Protein Name
- Serum paraoxonase/arylesterase 1
- Sample Data
- Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.041 0.092 0.141 0.23 0.422 0.768 1.348 2.377 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.041 0.092 0.141 0.23 0.422 0.768 1.348 2.377 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.041 0.092 0.141 0.23 0.422 0.768 1.348 2.377 O.D. 0.041 0.092 0.141 0.23 0.422 0.768 1.348 2.377
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
