- Catalog number
- EK1144
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Tumor necrosis factor receptor superfamily member 13B, also known as TNFRSF13B or more commonly as TACI, is a transmembrane receptor protein found predominantly on the surface of B cells, which are an important part of the immune system. TACI controls T cell-independent B cell antibody responses, isotype switching, and B cell homeostasis. TACI is a lymphocyte-specific member of the tumor necrosis factor (TNF) receptor superfamily. It was originally discovered because of its ability to interact with calcium-modulator and cyclophilin ligand (CAML) . TACI was later found to play a crucial role in humoral immunity by interacting with two members of the TNF family: BAFF and APRIL. These proteins signal through TACI inducing activation of several transcription factors including NFAT, AP-1, and NF-kappa-B which then modulate cellular activities. Defects in the function of TACI can lead to immune system diseases.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 88 91 89 95 90 2.68 2.9% Sample 2 327 381 369 367 361 20.34 5.6% Sample 3 1327 1381 1420 1248 1344 64.51 4.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 88 91 89 95 90 2.68 2.9% Sample 1 88 91 89 95 90 2.68 2.9% Sample 2 327 381 369 367 361 20.34 5.6% Sample 2 327 381 369 367 361 20.34 5.6% Sample 3 1327 1381 1420 1248 1344 64.51 4.7% Sample 3 1327 1381 1420 1248 1344 64.51 4.7%
- Synonyms
- Tumor necrosis factor receptor superfamily member 13B; Transmembrane activator and CAML interactor; CD267; Tnfrsf13b; Taci
- Gene Name
- TNFRSF13B
- Gene ID
- 57916
- UniProt
- Q9ET35
- Reactivity
- Mouse
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: A2-C128
- Capture Antibody
- Monoclonal antibody from rat
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 46.9 pg/mL - 3,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 88 327 1327 96 311 1224 Standard deviation 4.92 22.89 74.31 7.1 22.7 88.12 CV (%) 7%% 7% 5.6% 7.4% 7.3% 7.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 88 327 1327 96 311 1224 Standard deviation 4.92 22.89 74.31 7.1 22.7 88.12 CV (%) 7%% 7% 5.6% 7.4% 7.3% 7.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 88 327 1327 96 311 1224 Mean (pg/ml) 88 327 1327 96 311 1224 Standard deviation 4.92 22.89 74.31 7.1 22.7 88.12 Standard deviation 4.92 22.89 74.31 7.1 22.7 88.12 CV (%) 7%% 7% 5.6% 7.4% 7.3% 7.2% CV (%) 7%% 7% 5.6% 7.4% 7.3% 7.2%
- Sensitivity
- <10 pg/mL
- Function
- Receptor for TNFSF13/APRIL and TNFSF13B/TALL1/BAFF/BLYS that binds both ligands with similar high affinity. Mediates calcineurin-dependent activation of NF-AT, as well as activation of NF-kappa-B and AP-1. Involved in the stimulation of B- and T- cell function and the regulation of humoral immunity (By similarity) . .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 26947 MW
- Specificity
- Natural and recombinant mouse TNFRSF13B
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Tumor necrosis factor receptor superfamily member 13B
- Subcellular Location
- Membrane ; Single-pass type III membrane protein .
- Protein Name
- Tumor necrosis factor receptor superfamily member 13B
- Sample Data
- Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 O.D. 0.04 0.137 0.259 0.473 0.807 1.307 1.783 2.01 Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 O.D. 0.04 0.137 0.259 0.473 0.807 1.307 1.783 2.01 Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 O.D. 0.04 0.137 0.259 0.473 0.807 1.307 1.783 2.01 O.D. 0.04 0.137 0.259 0.473 0.807 1.307 1.783 2.01
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
