- Catalog number
- EK1146
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- CD163 (Cluster of Differentiation 163) is a human protein encoded by the CD163 gene. It has also been shown to mark cells of monocyte/macrophage lineage. CD163, a member of the scavenger receptor cysteine-rich (SRCR) superfamily, is exclusively expressed by monocytes and macrophages. Using FISH, somatic cell hybrid analysis, and radiation hybrid analysis, CD163 gene was mapped the to chromosome 12p13.3. CD163 is upregulated in a large range of diseases inflammatory diseases including type 2 diabetes, macrophage activation sickness, Tangier's disease, reumatoid arthritis etc.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1576 1541 1503 1533 1538 25.99 1.6% Sample 2 5152 5777 5436 5672 5509 240.4 4.3% Sample 3 22633 24816 23376 25733 24139 1209.27 5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1576 1541 1503 1533 1538 25.99 1.6% Sample 1 1576 1541 1503 1533 1538 25.99 1.6% Sample 2 5152 5777 5436 5672 5509 240.4 4.3% Sample 2 5152 5777 5436 5672 5509 240.4 4.3% Sample 3 22633 24816 23376 25733 24139 1209.27 5% Sample 3 22633 24816 23376 25733 24139 1209.27 5%
- Synonyms
- Scavenger receptor cysteine-rich type 1 protein M130; CD163; Soluble CD163; sCD163; Cd163; M130
- Gene Name
- CD163
- Gene ID
- 93671
- UniProt
- Q2VLH6
- Reactivity
- Mouse
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: V39-T1045
- Capture Antibody
- Monoclonal antibody from rat
- Detection Antibody
- Polyclonal antibody from goat
- Tissue Specificity
- Expressed in monocytes and mature macrophages such as Kupffer cells in the liver, red pulp macrophages in the spleen and mesenteric lymph nodes. .
- Applications
- ELISA
- Field of Research
- Atherosclerosis, Cardiovascular, Cell Type Markers, Immunology, Innate Immunity, Macrophage/Inflammation, Vascular Inflammation
- Standard
- 50 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin) .
- Detection Range
- 0.78 ng/mL - 50 ng/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1576 5152 22633 1548 5417 22589 Standard deviation 122.92 221.53 1606.94 131.58 254.59 1784.53 CV (%) 7.8% 4.3% 7.1% 8.5% 4.7% 7.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1576 5152 22633 1548 5417 22589 Standard deviation 122.92 221.53 1606.94 131.58 254.59 1784.53 CV (%) 7.8% 4.3% 7.1% 8.5% 4.7% 7.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 1576 5152 22633 1548 5417 22589 Mean (pg/ml) 1576 5152 22633 1548 5417 22589 Standard deviation 122.92 221.53 1606.94 131.58 254.59 1784.53 Standard deviation 122.92 221.53 1606.94 131.58 254.59 1784.53 CV (%) 7.8% 4.3% 7.1% 8.5% 4.7% 7.9% CV (%) 7.8% 4.3% 7.1% 8.5% 4.7% 7.9%
- Sensitivity
- <20 pg/mL
- Function
- Involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium- dependent and pH-dependent manner. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1 (By similarity) . .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 120921 MW
- Specificity
- Natural and recombinant mouse CD163
- Anticoagulant
- Heparin
- Gene Name Synonym
- Scavenger receptor cysteine-rich type 1 protein M130
- Subcellular Location
- Soluble CD163: Secreted .
- Protein Name
- Scavenger receptor cysteine-rich type 1 protein M130
- Sample Data
- Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.08 0.126 0.158 0.204 0.312 0.521 1.018 1.894 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.08 0.126 0.158 0.204 0.312 0.521 1.018 1.894 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.08 0.126 0.158 0.204 0.312 0.521 1.018 1.894 O.D. 0.08 0.126 0.158 0.204 0.312 0.521 1.018 1.894
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
