- Catalog number
- EK1173
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Heparin-binding growth factor 1 is a protein that in humans is encoded by the FGF1 gene. The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis. The FGF1 gene was mapped to chromosome 5q31.3-q33.2 by in situ hybridization.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 101 102 89 88 95 6.51 6.8% Sample 2 288 252 243 289 268 20.74 7.7% Sample 3 887 811 757 818 818 46.18 5.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 101 102 89 88 95 6.51 6.8% Sample 1 101 102 89 88 95 6.51 6.8% Sample 2 288 252 243 289 268 20.74 7.7% Sample 2 288 252 243 289 268 20.74 7.7% Sample 3 887 811 757 818 818 46.18 5.6% Sample 3 887 811 757 818 818 46.18 5.6%
- Synonyms
- Fibroblast growth factor 1; FGF-1; Acidic fibroblast growth factor; aFGF; Heparin-binding growth factor 1; HBGF-1; Fgf1; Fgf-1, Fgfa
- Gene Name
- FGF1
- Gene ID
- 25317
- UniProt
- P61149
- Reactivity
- Rat
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: E.coli; Immunogen sequence: F16-D155
- Capture Antibody
- Monoclonal antibody from mouse
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Field of Research
- Angiogenesis, Cancer, Cardiovascular, Growth Factors, Growth Factors/Hormones, Signal Transduction
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 31.2 pg/mL - 2,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 101 288 887 96 312 856 Standard deviation 7.97 19.58 37.25 9.12 26.2 37.66 CV (%) 7.9% 6.8% 4.2% 9.5% 8.4% 4.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 101 288 887 96 312 856 Standard deviation 7.97 19.58 37.25 9.12 26.2 37.66 CV (%) 7.9% 6.8% 4.2% 9.5% 8.4% 4.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 101 288 887 96 312 856 Mean (pg/ml) 101 288 887 96 312 856 Standard deviation 7.97 19.58 37.25 9.12 26.2 37.66 Standard deviation 7.97 19.58 37.25 9.12 26.2 37.66 CV (%) 7.9% 6.8% 4.2% 9.5% 8.4% 4.4% CV (%) 7.9% 6.8% 4.2% 9.5% 8.4% 4.4%
- Sensitivity
- <10 pg/mL
- Function
- Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 17418 MW
- Specificity
- Natural and recombinant rat FGF1
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Fibroblast growth factor 1
- Subcellular Location
- Secreted . Cytoplasm . Cytoplasm, cell cortex . Cytoplasm, cytosol . Nucleus . Lacks a cleavable signal sequence. Within the cytoplasm, it is transported to the cell membrane and then secreted by a non- classical pathway that requires Cu (2+) ions and S100A13. Secreted in a complex with SYT1 (By similarity) . Binding of exogenous FGF1 to FGFR facilitates endocytosis followed by translocation of FGF1 across endosomal membrane into the cytosol. Nuclear import from the cytosol requires the classical nuclear import machinery, involving proteins KPNA1 and KPNB1, as well as LRRC59 (By similarity) . .
- Protein Name
- Fibroblast growth factor 1
- Sample Data
- Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.065 0.244 0.355 0.508 0.739 1.093 1.586 2.316 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.065 0.244 0.355 0.508 0.739 1.093 1.586 2.316 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.065 0.244 0.355 0.508 0.739 1.093 1.586 2.316 O.D. 0.065 0.244 0.355 0.508 0.739 1.093 1.586 2.316
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
