- Catalog number
- EK1176
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Tumor necrosis factor ligand superfamily member 12 also known as TNF-related weak inducer of apoptosis (TWEAK) is a protein that in humans is encoded by the TNFSF12 gene. The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This protein is a ligand for the FN14/TWEAKR receptor. This cytokine has overlapping signaling functions with TNF, but displays a much wider tissue distribution. This cytokine can induce apoptosis via multiple pathways of cell death in a cell type-specific manner. This cytokine is also found to promote proliferation and migration of endothelial cells, and thus acts as a regulator ofangiogenesis. The TNFSF12 gene lies 878 bp upstream of the putative transcriptional start site of the TNFSF13 gene on chromosome 17p13.1.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 39 40 42 37 39 1.8 4.6% Sample 2 192 212 192 199 198 8.16 4.1% Sample 3 433 494 494 501 480 27.57 5.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 39 40 42 37 39 1.8 4.6% Sample 1 39 40 42 37 39 1.8 4.6% Sample 2 192 212 192 199 198 8.16 4.1% Sample 2 192 212 192 199 198 8.16 4.1% Sample 3 433 494 494 501 480 27.57 5.7% Sample 3 433 494 494 501 480 27.57 5.7%
- Synonyms
- Tumor necrosis factor ligand superfamily member 12; TNF-related weak inducer of apoptosis; TWEAK; Tumor necrosis factor ligand superfamily member 12, membrane form; Tumor necrosis factor ligand superfamily member 12, secreted form; Tnfsf12
- Gene Name
- TNFSF12
- Gene ID
- 21944
- UniProt
- O54907
- Reactivity
- Mouse
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: E.coli; Immunogen sequence: S94-H249
- Capture Antibody
- Polyclonal antibody from goat
- Detection Antibody
- Polyclonal antibody from goat
- Tissue Specificity
- Widely expressed.
- Applications
- ELISA
- Field of Research
- Apoptosis, Cancer, Cell Biology, Cell Death, Growth Factors, Invasion/Microenvironment
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 15.6 pg/mL - 1,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 39 192 433 42 205 410 Standard deviation 1.83 9.79 18.61 2.47 10.45 22.14 CV (%) 4.7% 5.4% 4.3% 5.9% 5.4% 5.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 39 192 433 42 205 410 Standard deviation 1.83 9.79 18.61 2.47 10.45 22.14 CV (%) 4.7% 5.4% 4.3% 5.9% 5.4% 5.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 39 192 433 42 205 410 Mean (pg/ml) 39 192 433 42 205 410 Standard deviation 1.83 9.79 18.61 2.47 10.45 22.14 Standard deviation 1.83 9.79 18.61 2.47 10.45 22.14 CV (%) 4.7% 5.4% 4.3% 5.9% 5.4% 5.4% CV (%) 4.7% 5.4% 4.3% 5.9% 5.4% 5.4%
- Sensitivity
- <10 pg/mL
- Function
- Binds to FN14 and possibly also to TNRFSF12/APO3. Weak inducer of apoptosis in some cell types. Mediates NF-kappa-B activation. Promotes angiogenesis and the proliferation of endothelial cells. Also involved in induction of inflammatory cytokines. Promotes IL8 secretion (By similarity) . .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 27441 MW
- Specificity
- Natural and recombinant mouse TNFSF12
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Tumor necrosis factor ligand superfamily member 12
- Subcellular Location
- Cell membrane ; Single-pass type II membrane protein .
- Protein Name
- Tumor necrosis factor ligand superfamily member 12
- Sample Data
- Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.003 0.075 0.142 0.259 0.464 0.865 1.359 1.887 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.003 0.075 0.142 0.259 0.464 0.865 1.359 1.887 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.003 0.075 0.142 0.259 0.464 0.865 1.359 1.887 O.D. 0.003 0.075 0.142 0.259 0.464 0.865 1.359 1.887
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
