- Catalog number
- EK1179
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Sclerostin is a protein that in humans is encoded by the SOST gene. Sclerostin is a secreted glycoprotein with a C-terminal cysteine knot-like (CTCK) domain and sequence similarity to the DAN (differential screening-selected gene aberrative in neuroblastoma) family of bone morphogenetic protein (BMP) antagonists. Sclerostin is produced by the osteocyte and has anti-anabolic effects on bone formation. The SOST gene maps to chromosome 17q12-q21
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 30 31 32 30 30 0.82 2.7% Sample 2 190 179 179 181 182 4.54 2.4% Sample 3 438 503 469 495 476 25.41 5.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 30 31 32 30 30 0.82 2.7% Sample 1 30 31 32 30 30 0.82 2.7% Sample 2 190 179 179 181 182 4.54 2.4% Sample 2 190 179 179 181 182 4.54 2.4% Sample 3 438 503 469 495 476 25.41 5.3% Sample 3 438 503 469 495 476 25.41 5.3%
- Synonyms
- Sclerostin; Sost
- Gene Name
- SOST
- Gene ID
- 74499
- UniProt
- Q99P68
- Reactivity
- Mouse
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: Q24-Y211
- Capture Antibody
- Polyclonal antibody from goat
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Field of Research
- Cytoskeleton/ECM, Developmental Biology, Extracellular Matrix, Growth Factors/Hormones, Metabolism, Organogenesis, Secreted, Signal Transduction, Signaling Pathways, Skeletal Development, Stem Cells, Structures
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 15.6 pg/mL - 1,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 30 190 438 28 192 398 Standard deviation 2.04 13.49 22.77 2.24 15.55 28.05 CV (%) 6.8% 7.1% 5.2% 8% 8.1% 7.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 30 190 438 28 192 398 Standard deviation 2.04 13.49 22.77 2.24 15.55 28.05 CV (%) 6.8% 7.1% 5.2% 8% 8.1% 7.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 30 190 438 28 192 398 Mean (pg/ml) 30 190 438 28 192 398 Standard deviation 2.04 13.49 22.77 2.24 15.55 28.05 Standard deviation 2.04 13.49 22.77 2.24 15.55 28.05 CV (%) 6.8% 7.1% 5.2% 8% 8.1% 7.1% CV (%) 6.8% 7.1% 5.2% 8% 8.1% 7.1%
- Sensitivity
- <10 pg/mL
- Function
- Negative regulator of bone growth that acts through inhibition of Wnt signaling and bone formation. .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 23443 MW
- Specificity
- Natural and recombinant mouse Sclerostin
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Sclerostin
- Subcellular Location
- Secreted, extracellular space, extracellular matrix .
- Sequence Similarities
- Belongs to the sclerostin family.
- Protein Name
- Sclerostin
- Sample Data
- Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.013 0.081 0.155 0.28 0.542 0.976 1.539 1.966 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.013 0.081 0.155 0.28 0.542 0.976 1.539 1.966 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.013 0.081 0.155 0.28 0.542 0.976 1.539 1.966 O.D. 0.013 0.081 0.155 0.28 0.542 0.976 1.539 1.966
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
