- Catalog number
- EK1185
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- OX-2 membrane glycoprotein, also known as CD200 is a human protein encoded by the CD200 gene. This gene is mapped to 3q13.2. The protein encoded by this gene is a type-1 membrane glycoprotein, which contains two immunoglobulin domains, and thus belongs to the immunoglobulin superfamily. Studies of the related genes in mouse and rat suggest that this gene may regulate myeloid cell activity and delivers an inhibitory signal for the macrophage lineage in diverse tissues. It is suggested that CD200-CD200R signaling plays a central role in macrophage fusion and osteoclast formation.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 76 78 75 70 74 2.94 3.9% Sample 2 242 250 227 231 237 9.06 3.8% Sample 3 1012 965 929 851 939 58.84 6.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 76 78 75 70 74 2.94 3.9% Sample 1 76 78 75 70 74 2.94 3.9% Sample 2 242 250 227 231 237 9.06 3.8% Sample 2 242 250 227 231 237 9.06 3.8% Sample 3 1012 965 929 851 939 58.84 6.2% Sample 3 1012 965 929 851 939 58.84 6.2%
- Synonyms
- OX-2 membrane glycoprotein; MRC OX-2 antigen; CD200; Cd200; Mox2
- Gene Name
- CD200
- Gene ID
- 17470
- UniProt
- O54901
- Reactivity
- Mouse
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: Q31-G232
- Capture Antibody
- Polyclonal antibody from goat
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Field of Research
- Adaptive Immunity, Atherosclerosis, Cardiovascular, Immunology, T Cells, Vascular Inflammation
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 31.2 pg/mL - 2,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 76 242 1012 83 236 1031 Standard deviation 5.32 17.66 77.92 5.89 21.24 96.91 CV (%) 7% 7.3% 7.7% 7.1% 9% 9.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 76 242 1012 83 236 1031 Standard deviation 5.32 17.66 77.92 5.89 21.24 96.91 CV (%) 7% 7.3% 7.7% 7.1% 9% 9.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 76 242 1012 83 236 1031 Mean (pg/ml) 76 242 1012 83 236 1031 Standard deviation 5.32 17.66 77.92 5.89 21.24 96.91 Standard deviation 5.32 17.66 77.92 5.89 21.24 96.91 CV (%) 7% 7.3% 7.7% 7.1% 9% 9.4% CV (%) 7% 7.3% 7.7% 7.1% 9% 9.4%
- Sensitivity
- <10 pg/mL
- Function
- Costimulates T-cell proliferation. May regulate myeloid cell activity in a variety of tissues (By similarity) . .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 31256 MW
- Specificity
- Natural and recombinant mouse CD200
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- OX-2 membrane glycoprotein
- Subcellular Location
- Cell membrane; Single-pass type I membrane protein.
- Sequence Similarities
- Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
- Protein Name
- OX-2 membrane glycoprotein
- Sample Data
- Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.011 0.058 0.102 0.18 0.325 0.582 1.153 1.974 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.011 0.058 0.102 0.18 0.325 0.582 1.153 1.974 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.011 0.058 0.102 0.18 0.325 0.582 1.153 1.974 O.D. 0.011 0.058 0.102 0.18 0.325 0.582 1.153 1.974
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
