- Catalog number
- EK1187
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Periostin, also known as OSF2, is a protein that in humans is encoded by the POSTN gene. The International Radiation Hybrid Mapping Consortium mapped the POSTN gene to chromosome 13. Periostin functions as a ligand for alpha-V/beta-3 and alpha-V/ beta-5 integrins to support adhesion and migration of epithelial cells. It is found that periostin was overexpressed by the majority of human primary breast cancers examined. After myocardial infarction, periostin-induced cardiomyocyte cell cycle reentry and mitosis were associated with improved ventricular remodeling and myocardial function, reduced fibrosis and infarct size, and increase angiogenesis.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 109 109 111 107 109 1.41 1.2% Sample 2 795 840 784 883 825 39.27 4.7% Sample 3 2510 2434 2494 2308 2436 79.41 3.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 109 109 111 107 109 1.41 1.2% Sample 1 109 109 111 107 109 1.41 1.2% Sample 2 795 840 784 883 825 39.27 4.7% Sample 2 795 840 784 883 825 39.27 4.7% Sample 3 2510 2434 2494 2308 2436 79.41 3.2% Sample 3 2510 2434 2494 2308 2436 79.41 3.2%
- Synonyms
- Periostin; PN; Osteoblast-specific factor 2; OSF-2; Postn; Osf2
- Gene Name
- POSTN
- Gene ID
- 50706
- UniProt
- Q62009
- Reactivity
- Mouse
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: N24-Q836
- Capture Antibody
- Monoclonal antibody from rat
- Detection Antibody
- Polyclonal antibody from goat
- Tissue Specificity
- Preferentially expressed in periosteum and periodontal ligament. Also expressed in the developing and adult heart. .
- Applications
- ELISA
- Field of Research
- Atherosclerosis, Cancer, Cardiogenesis, Cardiovascular, Cell Adhesion Molecules, Cell Type Markers, Cytoskeleton/ECM, Extracellular Matrix, Invasion/Microenvironment, Signal Transduction, Structures, Tags & Cell Markers, Vascular Inflammation
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 93.7 pg/mL - 6,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 109 7119 2510 119 740 2598 Standard deviation 6.97 53.26 110.44 8.33 62.9 119.5 CV (%) 6.4% 6.7% 4.4% 7% 8.5% 4.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 109 7119 2510 119 740 2598 Standard deviation 6.97 53.26 110.44 8.33 62.9 119.5 CV (%) 6.4% 6.7% 4.4% 7% 8.5% 4.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 109 7119 2510 119 740 2598 Mean (pg/ml) 109 7119 2510 119 740 2598 Standard deviation 6.97 53.26 110.44 8.33 62.9 119.5 Standard deviation 6.97 53.26 110.44 8.33 62.9 119.5 CV (%) 6.4% 6.7% 4.4% 7% 8.5% 4.6% CV (%) 6.4% 6.7% 4.4% 7% 8.5% 4.6%
- Sensitivity
- <10 pg/mL
- Function
- Induces cell attachment and spreading and plays a role in cell adhesion. May play a role in extracellular matrix mineralization. Enhances incorporation of BMP1 in the fibronectin matrix of connective tissues, and subsequent proteolytic activation of lysyl oxidase LOX. .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 93144 MW
- Specificity
- Natural and recombinant mouse Periostin
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Periostin
- Subcellular Location
- Golgi apparatus. Secreted, extracellular space, extracellular matrix. Colocalizes with BMP1 in the Golgi.
- Sequence Similarities
- Contains 1 EMI domain.
- Protein Name
- Periostin
- Sample Data
- Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.043 0.099 0.152 0.269 0.454 0.833 1.44 2.129 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.043 0.099 0.152 0.269 0.454 0.833 1.44 2.129 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.043 0.099 0.152 0.269 0.454 0.833 1.44 2.129 O.D. 0.043 0.099 0.152 0.269 0.454 0.833 1.44 2.129
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
