- Catalog number
- EK1196
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- GAS6 (Growth arrest-specific 6), also known as AXLLG or AXSF, is a human gene coding for the Gas6 protein. It is mapped to 13q34. GAS6 was originally found as a gene upregulated by growth arrested fibroblasts. This gene product is a gamma-carboxyglutamic acid (Gla) -containing protein which thought to be involved in the stimulation of cell proliferation, and may play a role in thrombosis. What's more, the product of the GAS6 gene, a ligand for the AXL, MER, and TYRO3 tyrosine kinase receptors, is a vitamin K-dependent protein, structurally related to anticoagulant protein S but lacking its anticoagulant activity. This protein also shows great importance in the cardiovascular system.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 50 58 58 56 55 3.27 5.9% Sample 2 232 221 213 230 224 7.58 3.3% Sample 3 1017 985 986 943 982 26.3 2.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 50 58 58 56 55 3.27 5.9% Sample 1 50 58 58 56 55 3.27 5.9% Sample 2 232 221 213 230 224 7.58 3.3% Sample 2 232 221 213 230 224 7.58 3.3% Sample 3 1017 985 986 943 982 26.3 2.6% Sample 3 1017 985 986 943 982 26.3 2.6%
- Synonyms
- Growth arrest-specific protein 6; GAS-6; AXL receptor tyrosine kinase ligand; Gas6
- Gene Name
- GAS6
- Gene ID
- 14456
- UniProt
- Q61592
- Reactivity
- Mouse
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: D115-P673
- Capture Antibody
- Monoclonal antibody from rat
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Field of Research
- Receptor Tyrosine Kinases, Signal Transduction, Stem Cells, Tyrosine Kinases
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 31.2 pg/mL - 2,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 50 232 1017 51 224 949 Standard deviation 2 14.15 57.96 2.55 14.33 55.79 CV (%) 4% 6.1% 5.7% 5% 6.4% 5.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 50 232 1017 51 224 949 Standard deviation 2 14.15 57.96 2.55 14.33 55.79 CV (%) 4% 6.1% 5.7% 5% 6.4% 5.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 50 232 1017 51 224 949 Mean (pg/ml) 50 232 1017 51 224 949 Standard deviation 2 14.15 57.96 2.55 14.33 55.79 Standard deviation 2 14.15 57.96 2.55 14.33 55.79 CV (%) 4% 6.1% 5.7% 5% 6.4% 5.9% CV (%) 4% 6.1% 5.7% 5% 6.4% 5.9%
- Sensitivity
- <10 pg/mL
- Function
- Ligand for tyrosine-protein kinase receptors AXL, TYRO3 and MER whose signaling is implicated in cell growth and survival, cell adhesion and cell migration. GAS6/AXL signaling plays a role in various processes such as endothelial cell survival during acidification by preventing apoptosis, optimal cytokine signaling during human natural killer cell development, hepatic regeneration, gonadotropin-releasing hormone neuron survival and migration, platelet activation, or regulation of thrombotic responses. .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 74610 MW
- Specificity
- Natural and recombinant mouse GAS6
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Growth arrest-specific protein 6
- Subcellular Location
- Secreted .
- Sequence Similarities
- Contains 4 EGF-like domains.
- Protein Name
- Growth arrest-specific protein 6
- Sample Data
- Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.02 0.071 0.124 0.208 0.393 0.743 1.395 2.392 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.02 0.071 0.124 0.208 0.393 0.743 1.395 2.392 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.02 0.071 0.124 0.208 0.393 0.743 1.395 2.392 O.D. 0.02 0.071 0.124 0.208 0.393 0.743 1.395 2.392
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
