- Catalog number
- EK1200
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- LIFR, also known as CD118 (Cluster of Differentiation 118), is a subunit of a receptor for leukemia inhibitory factor. This gene encodes a protein that belongs to the type I cytokine receptor family. It is mapped to 5p31.1. The LIF receptor (LIFR) is the low-affinity binding chain that, together with the high-affinity converter subunit gp130, forms a high-affinity receptor complex that mediates the action of the leukemia-inhibitory factor. LIF is a polyfunctional cytokine that affects the differentiation, survival, and proliferation of a wide variety of cells in the adult and the embryo. Mutations in this gene cause Schwartz-Jampel syndrome type 2, a disease belonging to the group of the bent-bone dysplasias. A translocation that involves the promoter of this gene, togerther with the pleiomorphic adenoma gene 1, is associated with salivary gland pleiomorphic adenoma, a common type of benign epithelial tumor of the salivary gland.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 289 279 327 287 295 18.56 6.2% Sample 2 1861 1799 1747 1540 1736 120.54 6.9% Sample 3 6421 5493 5855 5885 5913 331.13 5.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 289 279 327 287 295 18.56 6.2% Sample 1 289 279 327 287 295 18.56 6.2% Sample 2 1861 1799 1747 1540 1736 120.54 6.9% Sample 2 1861 1799 1747 1540 1736 120.54 6.9% Sample 3 6421 5493 5855 5885 5913 331.13 5.6% Sample 3 6421 5493 5855 5885 5913 331.13 5.6%
- Synonyms
- Leukemia inhibitory factor receptor; LIF receptor; LIF-R; CD118; LIFR
- Gene Name
- LIFR
- Gene ID
- 3977
- UniProt
- P42702
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: Q45-S833
- Capture Antibody
- Polyclonal antibody from goat
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Field of Research
- Cell Type Markers, Cytokines, Developmental Biology, Embryogenesis, Embryonic Stem Cells, Immunology, Innate Immunity, Intracellular, Stem Cells, Surface Molecules
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 156 pg/mL - 10,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 289 1861 6421 261 1769 6236 Standard deviation 16.76 91.18 276.1 16.7 107.9 386.63 CV (%) 5.8% 4.9% 4.3% 6.4% 6.1% 6.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 289 1861 6421 261 1769 6236 Standard deviation 16.76 91.18 276.1 16.7 107.9 386.63 CV (%) 5.8% 4.9% 4.3% 6.4% 6.1% 6.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 289 1861 6421 261 1769 6236 Mean (pg/ml) 289 1861 6421 261 1769 6236 Standard deviation 16.76 91.18 276.1 16.7 107.9 386.63 Standard deviation 16.76 91.18 276.1 16.7 107.9 386.63 CV (%) 5.8% 4.9% 4.3% 6.4% 6.1% 6.2% CV (%) 5.8% 4.9% 4.3% 6.4% 6.1% 6.2%
- Sensitivity
- <10 pg/mL
- Function
- Signal-transducing molecule. May have a common pathway with IL6ST. The soluble form inhibits the biological activity of LIF by blocking its binding to receptors on target cells.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 123743 MW
- Specificity
- Natural and recombinant human LIFR
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Leukemia inhibitory factor receptor
- Subcellular Location
- Isoform 1: Cell membrane; Single-pass type I membrane protein.
- Sequence Similarities
- Belongs to the type I cytokine receptor family. Type 2 subfamily.
- Protein Name
- Leukemia inhibitory factor receptor
- Sample Data
- Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.008 0.038 0.093 0.195 0.381 0.793 1.456 2.357 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.008 0.038 0.093 0.195 0.381 0.793 1.456 2.357 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.008 0.038 0.093 0.195 0.381 0.793 1.456 2.357 O.D. 0.008 0.038 0.093 0.195 0.381 0.793 1.456 2.357
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
