- Catalog number
- EK1201
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- MFGE8 (Milk Fat Globule-Egf Factor 8), also called as Lactadherin or SED1, is a protein which in humans is encoded by the MFGE8 gene. Mfge8 is secreted protein found in vertebrates, including mammals as well as birds. By fluorescence in situ hybridization, the MFGE8 gene was mapped to chromosome 15q25. MFGE8 is a factor that links apoptotic cells to phagocytes. It specifically bound to apoptotic cells by recognizing aminophospholipids such as phosphatidylserine. MFGE8, when engaged by phospholipids, bound to cells via its RGD motif. It bound particularly strongly to cells expressing alpha-V-beta-3 integrin. It showed that Mfge8 was expressed in intestinal lamina propria macrophages in mice. Using a wound-healing assay, they showed that Mfge8 promoted migration of intestinal epithelial cells through a PKC-epsilon (PRKCE) -dependent mechanism.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 100 98 103 96 99 2.58 2.6% Sample 2 660 663 784 706 703 50.04 7.1% Sample 3 1196 1025 1141 1197 1139 70.01 6.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 100 98 103 96 99 2.58 2.6% Sample 1 100 98 103 96 99 2.58 2.6% Sample 2 660 663 784 706 703 50.04 7.1% Sample 2 660 663 784 706 703 50.04 7.1% Sample 3 1196 1025 1141 1197 1139 70.01 6.1% Sample 3 1196 1025 1141 1197 1139 70.01 6.1%
- Synonyms
- Lactadherin; Breast epithelial antigen BA46; HMFG; MFGM; Milk fat globule-EGF factor 8; MFG-E8; SED1; Lactadherin short form; Medin; MFGE8
- Gene Name
- MFGE8
- Gene ID
- 4240
- UniProt
- Q08431
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: L24-C387
- Capture Antibody
- Monoclonal antibody from mouse
- Detection Antibody
- Polyclonal antibody from goat
- Tissue Specificity
- Mammary epithelial cell surfaces and aortic media. Overexpressed in several carcinomas.
- Applications
- ELISA
- Field of Research
- Angiogenesis, Apoptosis, Cancer, Cardiovascular, Cell Biology, Cell Death, Metabolism, Metabolism Processes, Pathways and Processes
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum, plasma (heparin, EDTA), saliva, urine and human milk.
- Detection Range
- 62.5 pg/mL - 4,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 100 660 1196 101 717 1149 Standard deviation 5.8 36.96 90.89 8.28 41.58 102.26 CV (%) 5.8% 5.6% 7.6% 8.2% 5.8% 8.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 100 660 1196 101 717 1149 Standard deviation 5.8 36.96 90.89 8.28 41.58 102.26 CV (%) 5.8% 5.6% 7.6% 8.2% 5.8% 8.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 100 660 1196 101 717 1149 Mean (pg/ml) 100 660 1196 101 717 1149 Standard deviation 5.8 36.96 90.89 8.28 41.58 102.26 Standard deviation 5.8 36.96 90.89 8.28 41.58 102.26 CV (%) 5.8% 5.6% 7.6% 8.2% 5.8% 8.9% CV (%) 5.8% 5.6% 7.6% 8.2% 5.8% 8.9%
- Sensitivity
- <10 pg/mL
- Function
- Plays an important role in the maintenance of intestinal epithelial homeostasis and the promotion of mucosal healing. Promotes VEGF-dependent neovascularization (By similarity) . Contributes to phagocytic removal of apoptotic cells in many tissues. Specific ligand for the alpha-v/beta-3 and alpha-v/beta-5 receptors. Also binds to phosphatidylserine-enriched cell surfaces in a receptor-independent manner. Zona pellucida-binding protein which may play a role in gamete interaction. Binds specifically to rotavirus and inhibits its replication. .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 43123 MW
- Specificity
- Natural and recombinant human MFGE8
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Lactadherin
- Subcellular Location
- Membrane ; Peripheral membrane protein . Secreted .
- Sequence Similarities
- Contains 1 EGF-like domain.
- Protein Name
- Lactadherin
- Sample Data
- Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.006 0.06 0.129 0.256 0.442 0.829 1.384 1.979 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.006 0.06 0.129 0.256 0.442 0.829 1.384 1.979 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.006 0.06 0.129 0.256 0.442 0.829 1.384 1.979 O.D. 0.006 0.06 0.129 0.256 0.442 0.829 1.384 1.979
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
