- Catalog number
- EK1202
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Transforming growth factor, beta-induced, 68kDa, also known as TGFBI (initially called BIGH3, BIG-H3), is a protein which in humans is encoded by the TGFBI gene. It is mapped to 5q31.1. This gene encodes an RGD-containing protein that binds to type I, II and IV collagens. The RGD motif is found in many extracellular matrix proteins modulating cell adhesion and serves as a ligand recognition sequence for several integrins. This protein plays a role in cell-collagen interactions and may be involved in endochondrial bone formation in cartilage. The protein is induced by transforming growth factor-beta and acts to inhibit cell adhesion.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 154 145 147 157 150 4.91 3.2% Sample 2 388 396 437 439 415 23.18 5.5% Sample 3 1132 1229 1284 1106 1187 72.03 6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 154 145 147 157 150 4.91 3.2% Sample 1 154 145 147 157 150 4.91 3.2% Sample 2 388 396 437 439 415 23.18 5.5% Sample 2 388 396 437 439 415 23.18 5.5% Sample 3 1132 1229 1284 1106 1187 72.03 6% Sample 3 1132 1229 1284 1106 1187 72.03 6%
- Synonyms
- Transforming growth factor-beta-induced protein ig-h3; Beta ig-h3; Kerato-epithelin; RGD-containing collagen-associated protein; RGD-CAP; TGFBI; BIGH3
- Gene Name
- TGFBI
- Gene ID
- 7045
- UniProt
- Q15582
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: G24-H683
- Capture Antibody
- Monoclonal antibody from mouse
- Detection Antibody
- Polyclonal antibody from goat
- Tissue Specificity
- Highly expressed in the corneal epithelium. .
- Applications
- ELISA
- Field of Research
- Collagen, Cytoskeleton/ECM, Developmental Biology, ECM Proteins, Extracellular Matrix, Growth Factors/Hormones, Neuroscience, Organogenesis, Sensory System, Signal Transduction, Structures, Visual System
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 62.5 pg/mL - 4,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 154 388 1132 160 387 1210 Standard deviation 6.77 28.32 57.73 7.36 34.41 70.18 CV (%) 4.4% 7.3% 5.8% 4.6% 9.1% 5.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 154 388 1132 160 387 1210 Standard deviation 6.77 28.32 57.73 7.36 34.41 70.18 CV (%) 4.4% 7.3% 5.8% 4.6% 9.1% 5.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 154 388 1132 160 387 1210 Mean (pg/ml) 154 388 1132 160 387 1210 Standard deviation 6.77 28.32 57.73 7.36 34.41 70.18 Standard deviation 6.77 28.32 57.73 7.36 34.41 70.18 CV (%) 4.4% 7.3% 5.8% 4.6% 9.1% 5.8% CV (%) 4.4% 7.3% 5.8% 4.6% 9.1% 5.8%
- Sensitivity
- <10 pg/mL
- Function
- Binds to type I, II, and IV collagens. This adhesion protein may play an important role in cell-collagen interactions. In cartilage, may be involved in endochondral bone formation.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 74681 MW
- Specificity
- Natural and recombinant human TGFBI
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Transforming growth factor-beta-induced protein ig-h3
- Subcellular Location
- Secreted, extracellular space, extracellular matrix. May be associated both with microfibrils and with the cell surface.
- Sequence Similarities
- Contains 1 EMI domain.
- Protein Name
- Transforming growth factor-beta-induced protein ig-h3
- Sample Data
- Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.031 0.089 0.157 0.305 0.587 1.032 1.818 2.369 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.031 0.089 0.157 0.305 0.587 1.032 1.818 2.369 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.031 0.089 0.157 0.305 0.587 1.032 1.818 2.369 O.D. 0.031 0.089 0.157 0.305 0.587 1.032 1.818 2.369
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
