- Catalog number
- EK1205
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Cystatin B (CSTB), also called STFB, is a small protein that is a member of the superfamily of cysteine protease inhibitors. It has been isolated from human spleen and liver and its amino acid sequence has been fully determined. The cystatin B gene is located on 21q22.3. It is widely distributed and is localized mostly intracellularly, but has been found extracellularly. The protein is able to form a dimer stabilized by noncovalent forces, inhibiting papain and cathepsins l, h and b. Its role is thought to be as a protector against the proteinases leaking from lysosomes. A cystatin B multiprotein complex might have a specific cerebellar function, and that the loss of this function might contribute to the etiopathogenesis of EPM1. Upon differentiation to myotubes, CSTB becomes excluded from the nucleus and lysosomes, suggesting that the subcellular distribution of CSTB is dependent on the differentiation status of the cell.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 108 102 101 101 103 2.91 2.8% Sample 2 522 468 537 457 496 34.13 6.8% Sample 3 1824 1767 1982 1870 1860 78.94 4.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 108 102 101 101 103 2.91 2.8% Sample 1 108 102 101 101 103 2.91 2.8% Sample 2 522 468 537 457 496 34.13 6.8% Sample 2 522 468 537 457 496 34.13 6.8% Sample 3 1824 1767 1982 1870 1860 78.94 4.2% Sample 3 1824 1767 1982 1870 1860 78.94 4.2%
- Synonyms
- Cystatin-B; CPI-B; Liver thiol proteinase inhibitor; Stefin-B; CSTB; CST6, STFB
- Gene Name
- CSTB
- Gene ID
- 1476
- UniProt
- P04080
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: E.coli; Immunogen sequence: M2-F98
- Capture Antibody
- Monoclonal antibody from mouse
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Field of Research
- Cell Biology, Protease Inhibitors, Proteolysis/Ubiquitin
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum, plasma (heparin, EDTA), saliva, urine and human milk.
- Detection Range
- 62.5 pg/mL - 4,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 108 522 1824 110 532 1878 Standard deviation 7.77 32.88 131.32 9.57 40.96 153.99 CV (%) 7.2% 7.7% 7.2% 8.7% 7.7% 8.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 108 522 1824 110 532 1878 Standard deviation 7.77 32.88 131.32 9.57 40.96 153.99 CV (%) 7.2% 7.7% 7.2% 8.7% 7.7% 8.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 108 522 1824 110 532 1878 Mean (pg/ml) 108 522 1824 110 532 1878 Standard deviation 7.77 32.88 131.32 9.57 40.96 153.99 Standard deviation 7.77 32.88 131.32 9.57 40.96 153.99 CV (%) 7.2% 7.7% 7.2% 8.7% 7.7% 8.2% CV (%) 7.2% 7.7% 7.2% 8.7% 7.7% 8.2%
- Sensitivity
- <10 pg/mL
- Function
- This is an intracellular thiol proteinase inhibitor. Tightly binding reversible inhibitor of cathepsins L, H and B.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 11140 MW
- Specificity
- Natural and recombinant human Cystatin B
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Cystatin-B
- Subcellular Location
- Cytoplasm . Nucleus .
- Sequence Similarities
- Belongs to the cystatin family.
- Protein Name
- Cystatin-B
- Sample Data
- Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.029 0.125 0.213 0.338 0.622 1.054 1.863 2.552 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.029 0.125 0.213 0.338 0.622 1.054 1.863 2.552 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.029 0.125 0.213 0.338 0.622 1.054 1.863 2.552 O.D. 0.029 0.125 0.213 0.338 0.622 1.054 1.863 2.552
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
