- Catalog number
- EK1221
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- CD6 (Cluster of Differentiation 6) is a human protein encoded by the CD6 gene. It is mapped to 11q12.2. CD6 is expressed predominantly in peripheral T cells and mature medullary thymocytes. This gene encodes a protein found on the outer membrane of T-lymphocytes as well as some other immune cells. The encoded protein contains three scavenger receptor cysteine-rich (SRCR) domains and a binding site for an activated leukocyte cell adhesion molecule. The gene product is important for continuation of T cell activation. What's more, this gene may be associated with susceptibility to multiple sclerosis.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 255 257 218 221 237 18.29 7.7% Sample 2 1450 1421 1416 1269 1389 70.48 5% Sample 3 4911 4340 4503 4742 4624 218.85 4.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 255 257 218 221 237 18.29 7.7% Sample 1 255 257 218 221 237 18.29 7.7% Sample 2 1450 1421 1416 1269 1389 70.48 5% Sample 2 1450 1421 1416 1269 1389 70.48 5% Sample 3 4911 4340 4503 4742 4624 218.85 4.7% Sample 3 4911 4340 4503 4742 4624 218.85 4.7%
- Synonyms
- T-cell differentiation antigen CD6; T12; TP120; CD6; CD6
- Gene Name
- CD6
- Gene ID
- 923
- UniProt
- P30203
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: H18-E398
- Capture Antibody
- Polyclonal antibody from goat
- Detection Antibody
- Polyclonal antibody from goat
- Tissue Specificity
- Expressed by thymocytes, mature T-cells, a subset of B-cells known as B-1 cells, and by some cells in the brain.
- Applications
- ELISA
- Field of Research
- Adaptive Immunity, Hematopoietic Progenitors, Immunology, Lymphoid, Stem Cells, T Cells, T Lymphocytic Lineage
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 156 pg/mL - 10,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 255 1450 4911 250 1511 5268 Standard deviation 14.79 72.5 378.14 16.5 75.55 447.78 CV (%) 5.8% 5% 7.7% 6.6% 5% 8.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 255 1450 4911 250 1511 5268 Standard deviation 14.79 72.5 378.14 16.5 75.55 447.78 CV (%) 5.8% 5% 7.7% 6.6% 5% 8.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 255 1450 4911 250 1511 5268 Mean (pg/ml) 255 1450 4911 250 1511 5268 Standard deviation 14.79 72.5 378.14 16.5 75.55 447.78 Standard deviation 14.79 72.5 378.14 16.5 75.55 447.78 CV (%) 5.8% 5% 7.7% 6.6% 5% 8.5% CV (%) 5.8% 5% 7.7% 6.6% 5% 8.5%
- Sensitivity
- <10 pg/mL
- Function
- Involved in cell adhesion. Binds to CD166.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 71801 MW
- Specificity
- Natural and recombinant human CD6
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- T-cell differentiation antigen CD6
- Subcellular Location
- Cell membrane ; Single-pass type I membrane protein .
- Protein Name
- T-cell differentiation antigen CD6
- Sample Data
- Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.041 0.133 0.207 0.347 0.559 0.942 1.417 1.848 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.041 0.133 0.207 0.347 0.559 0.942 1.417 1.848 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.041 0.133 0.207 0.347 0.559 0.942 1.417 1.848 O.D. 0.041 0.133 0.207 0.347 0.559 0.942 1.417 1.848
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
