- Catalog number
- EK1228
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Junctional adhesion molecule A (JAM-A) is a protein that in humans is encoded by the F11R gene. It is mapped to 1q23.3. This gene is an immunoglobulin-like molecule that colocalizes with tight junctions in endothelium and epithelium and is also found on blood leukocytes and platelets. JAM-A plays an important role in the regulation of tight junction assembly in epithelia. In addition, it can act as a receptor for reovirus, a ligand for the integrin LFA1, involved in leukocyte transmigration, and a platelet receptor. JAM-A has a nonredundant role in controlling DC motility, trafficking to lymph nodes, and activation of specific immunity.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 74 76 83 81 78 3.64 4.6% Sample 2 243 243 259 220 241 13.89 5.7% Sample 3 871 854 977 972 918 56.34 6.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 74 76 83 81 78 3.64 4.6% Sample 1 74 76 83 81 78 3.64 4.6% Sample 2 243 243 259 220 241 13.89 5.7% Sample 2 243 243 259 220 241 13.89 5.7% Sample 3 871 854 977 972 918 56.34 6.1% Sample 3 871 854 977 972 918 56.34 6.1%
- Synonyms
- Junctional adhesion molecule A; JAM-A; Junctional adhesion molecule 1; JAM-1; CD321; F11r; Jam1, Jcam, Jcam1
- Gene Name
- F11R
- Gene ID
- 16456
- UniProt
- O88792
- Reactivity
- Mouse
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: K27-G238
- Capture Antibody
- Monoclonal antibody from rat
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Field of Research
- Atherosclerosis, Cardiovascular, Cell Adhesion, Cytoskeleton/ECM, Platelets, Signal Transduction, Tight Junctions, Vascular Inflammation
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 31.2 pg/mL - 2,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 74 243 871 67 225 792 Standard deviation 3.18 18.95 57.48 3.28 18.67 61.77 CV (%) 4.3% 7.8% 6.6% 4.9% 8.3% 7.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 74 243 871 67 225 792 Standard deviation 3.18 18.95 57.48 3.28 18.67 61.77 CV (%) 4.3% 7.8% 6.6% 4.9% 8.3% 7.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 74 243 871 67 225 792 Mean (pg/ml) 74 243 871 67 225 792 Standard deviation 3.18 18.95 57.48 3.28 18.67 61.77 Standard deviation 3.18 18.95 57.48 3.28 18.67 61.77 CV (%) 4.3% 7.8% 6.6% 4.9% 8.3% 7.8% CV (%) 4.3% 7.8% 6.6% 4.9% 8.3% 7.8%
- Sensitivity
- <10 pg/mL
- Function
- Seems to play a role in epithelial tight junction formation. Appears early in primordial forms of cell junctions and recruits PARD3. The association of the PARD6-PARD3 complex may prevent the interaction of PARD3 with JAM1, thereby preventing tight junction assembly. Plays a role in regulating monocyte transmigration involved in integrity of epithelial barrier. Involved in platelet activation. In case of orthoreovirus infection, serves as receptor for the virus (By similarity) . .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 32424 MW
- Specificity
- Natural and recombinant mouse JAM-A
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Junctional adhesion molecule A
- Subcellular Location
- Cell junction, tight junction . Cell membrane ; Single-pass type I membrane protein . Localized at tight junctions of both epithelial and endothelial cells.
- Sequence Similarities
- Belongs to the immunoglobulin superfamily.
- Protein Name
- Junctional adhesion molecule A
- Sample Data
- Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.075 0.265 0.352 0.524 0.786 1.196 1.802 2.603 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.075 0.265 0.352 0.524 0.786 1.196 1.802 2.603 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.075 0.265 0.352 0.524 0.786 1.196 1.802 2.603 O.D. 0.075 0.265 0.352 0.524 0.786 1.196 1.802 2.603
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
