- Catalog number
- EK1231
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Relaxin 1 is a member of relaxin-like peptide family. Relaxin gene maps to mouse chromosome 19 near D19Mit23. Relaxin is a peptide hormone produced by the corpora lutea of ovaries during pregnancy in many mammalian species, including man. Relaxin widens the pubic bone and facilitates labor, it also softens the cervix (cervical ripening), and relaxes the uterine musculature. However, its significance may reach much further. Relaxin affects collagen metabolism, inhibiting collagen synthesis and enhancing its breakdown by increasing matrix metalloproteinases. It also enhances angiogenesis and is a potent renal vasodilator.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 104 106 113 116 109 4.91 4.5% Sample 2 612 581 605 608 601 12.09 2% Sample 3 2407 2396 2116 2524 2360 149.94 6.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 104 106 113 116 109 4.91 4.5% Sample 1 104 106 113 116 109 4.91 4.5% Sample 2 612 581 605 608 601 12.09 2% Sample 2 612 581 605 608 601 12.09 2% Sample 3 2407 2396 2116 2524 2360 149.94 6.3% Sample 3 2407 2396 2116 2524 2360 149.94 6.3%
- Synonyms
- Prorelaxin 1; Relaxin B chain; Relaxin A chain; Rln1; Rln, Rlx
- Gene Name
- RLN1
- Gene ID
- 19773
- UniProt
- P47932
- Reactivity
- Mouse
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: E.coli; Immunogen sequence: R23-R54 (B) & E161-C185 (A)
- Capture Antibody
- Polyclonal from sheep
- Detection Antibody
- Polyclonal from sheep
- Applications
- ELISA
- Field of Research
- Cell Type Markers, Growth Factors/Hormones, Signal Transduction, Tags & Cell Markers
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin, EDTA) .
- Detection Range
- 78 pg/mL - 5,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 104 612 2407 108 616 2227 Standard deviation 4.16 47.12 154.04 7.71 59.13 144.75 CV (%) 4% 7.7% 6.4% 5.2% 9.6% 6.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 104 612 2407 108 616 2227 Standard deviation 4.16 47.12 154.04 7.71 59.13 144.75 CV (%) 4% 7.7% 6.4% 5.2% 9.6% 6.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 104 612 2407 108 616 2227 Mean (pg/ml) 104 612 2407 108 616 2227 Standard deviation 4.16 47.12 154.04 7.71 59.13 144.75 Standard deviation 4.16 47.12 154.04 7.71 59.13 144.75 CV (%) 4% 7.7% 6.4% 5.2% 9.6% 6.5% CV (%) 4% 7.7% 6.4% 5.2% 9.6% 6.5%
- Sensitivity
- <10 pg/mL
- Function
- Relaxin is an ovarian hormone that acts with estrogen to produce dilatation of the birth canal in many mammals.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 20571 MW
- Specificity
- Natural and recombinant mouse Relaxin 1
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Prorelaxin 1
- Subcellular Location
- Secreted.
- Protein Name
- Prorelaxin 1
- Sample Data
- Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.047 0.164 0.276 0.445 0.698 1.136 1.619 1.913 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.047 0.164 0.276 0.445 0.698 1.136 1.619 1.913 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.047 0.164 0.276 0.445 0.698 1.136 1.619 1.913 O.D. 0.047 0.164 0.276 0.445 0.698 1.136 1.619 1.913
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
