- Catalog number
- EK1233
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Trefoil factor 2, also known as SP or SML1, is a protein that in humans is encoded by the TFF2 gene. This gene belongs to trefoil family, and members of the trefoil family may protect the mucosa from insults, stabilize the mucus layer, and affect healing of the epithelium. This gene is mapped to 21q22.3. TFF2 gene consists of 4 exons spanning 5.1 kb, with exons 2 and 3 encoding 1 trefoil domain each, and it is the only gene encoding 2 TFF-domains on separate exons. It has been found that the distribution of TFF2 and pS2 suggests involvement in repair-enhancing mechanisms.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 170 167 181 164 170 6.42 3.7% Sample 2 615 653 697 611 644 34.71 5.3% Sample 3 1677 1609 1754 1816 1714 78.09 4.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 170 167 181 164 170 6.42 3.7% Sample 1 170 167 181 164 170 6.42 3.7% Sample 2 615 653 697 611 644 34.71 5.3% Sample 2 615 653 697 611 644 34.71 5.3% Sample 3 1677 1609 1754 1816 1714 78.09 4.5% Sample 3 1677 1609 1754 1816 1714 78.09 4.5%
- Synonyms
- Trefoil factor 2; Spasmolysin; Spasmolytic polypeptide; SP; TFF2; SML1
- Gene Name
- TFF2
- Gene ID
- 7032
- UniProt
- Q03403
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: E24-Y129
- Capture Antibody
- Monoclonal antibody from mouse
- Detection Antibody
- Polyclonal antibody from goat
- Tissue Specificity
- Stomach.
- Applications
- ELISA
- Field of Research
- Cell Biology
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 62.5 pg/mL - 4,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 170 615 1677 164 630 1585 Standard deviation 9.01 40.59 92.23 10.16 46.62 91.93 CV (%) 5.3% 6.6% 5.5% 6.2% 7.4% 5.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 170 615 1677 164 630 1585 Standard deviation 9.01 40.59 92.23 10.16 46.62 91.93 CV (%) 5.3% 6.6% 5.5% 6.2% 7.4% 5.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 170 615 1677 164 630 1585 Mean (pg/ml) 170 615 1677 164 630 1585 Standard deviation 9.01 40.59 92.23 10.16 46.62 91.93 Standard deviation 9.01 40.59 92.23 10.16 46.62 91.93 CV (%) 5.3% 6.6% 5.5% 6.2% 7.4% 5.8% CV (%) 5.3% 6.6% 5.5% 6.2% 7.4% 5.8%
- Sensitivity
- <10 pg/mL
- Function
- Inhibits gastrointestinal motility and gastric acid secretion. Could function as a structural component of gastric mucus, possibly by stabilizing glycoproteins in the mucus gel through interactions with carbohydrate side chains (By similarity) . .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 14284 MW
- Specificity
- Natural and recombinant human TFF2
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Trefoil factor 2
- Subcellular Location
- Secreted.
- Sequence Similarities
- Contains 2 P-type (trefoil) domains.
- Protein Name
- Trefoil factor 2
- Sample Data
- Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.008 0.083 0.134 0.234 0.46 0.804 1.467 2.004 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.008 0.083 0.134 0.234 0.46 0.804 1.467 2.004 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.008 0.083 0.134 0.234 0.46 0.804 1.467 2.004 O.D. 0.008 0.083 0.134 0.234 0.46 0.804 1.467 2.004
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
