- Catalog number
- EK1241
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Serine protease inhibitor Kazal-type 1, also called TATI or PSTI, is a protein that in humans is encoded by the SPINK1 gene. It is mapped to 5q32. SPINK1 gene contains 4 exons spanning approximately 7.5 kb. It was initially detected in urine of patients with ovarian cancer. This peptide is also produced by the mucosa of the gastrointestinal tract where it may protect the mucosal cells from proteolytic breakdown. The SPINK1 gene encodes pancreatic secretory trypsin inhibitor, which is secreted from pancreatic acinar cells into pancreatic juice. Its physiologic role was thought to be the prevention of trypsin-catalyzed premature activation of zymogens within the pancreas and the pancreatic duct. Mutations in this gene are associated with hereditary pancreatitis and tropical calcific pancreatitis.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 245 238 239 225 236 7.29 3% Sample 2 1862 1592 1831 1832 1779 108.82 6.1% Sample 3 5817 5596 5823 5918 5788 118.14 2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 245 238 239 225 236 7.29 3% Sample 1 245 238 239 225 236 7.29 3% Sample 2 1862 1592 1831 1832 1779 108.82 6.1% Sample 2 1862 1592 1831 1832 1779 108.82 6.1% Sample 3 5817 5596 5823 5918 5788 118.14 2% Sample 3 5817 5596 5823 5918 5788 118.14 2%
- Synonyms
- Pancreatic secretory trypsin inhibitor; Serine protease inhibitor Kazal-type 1; Tumor-associated trypsin inhibitor; TATI; SPINK1; PSTI
- Gene Name
- SPINK1
- Gene ID
- 6690
- UniProt
- P00995
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: E.coli; Immunogen sequence: D24-C79
- Capture Antibody
- Monoclonal antibody from mouse
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Field of Research
- Cell Biology, Protease Inhibitors, Proteolysis/Ubiquitin
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin) .
- Detection Range
- 156 pg/mL - 10,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 245 1862 5817 254 1784 6136 Standard deviation 16.9 137.22 418.82 17.78 148.07 468.33 CV (%) 6.9% 7.3% 7.2% 7% 8.3% 7.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 245 1862 5817 254 1784 6136 Standard deviation 16.9 137.22 418.82 17.78 148.07 468.33 CV (%) 6.9% 7.3% 7.2% 7% 8.3% 7.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 245 1862 5817 254 1784 6136 Mean (pg/ml) 245 1862 5817 254 1784 6136 Standard deviation 16.9 137.22 418.82 17.78 148.07 468.33 Standard deviation 16.9 137.22 418.82 17.78 148.07 468.33 CV (%) 6.9% 7.3% 7.2% 7% 8.3% 7.6% CV (%) 6.9% 7.3% 7.2% 7% 8.3% 7.6%
- Sensitivity
- <10 pg/mL
- Function
- This is a trypsin inhibitor, its physiological function is to prevent the trypsin-catalyzed premature activation of zymogens within the pancreas.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 8507 MW
- Specificity
- Natural and recombinant human SPINK1
- Anticoagulant
- Heparin
- Gene Name Synonym
- Pancreatic secretory trypsin inhibitor
- Subcellular Location
- Secreted.
- Protein Name
- Pancreatic secretory trypsin inhibitor
- Sample Data
- Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.07 0.104 0.147 0.223 0.394 0.724 1.237 1.9 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.07 0.104 0.147 0.223 0.394 0.724 1.237 1.9 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.07 0.104 0.147 0.223 0.394 0.724 1.237 1.9 O.D. 0.07 0.104 0.147 0.223 0.394 0.724 1.237 1.9
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
