- Catalog number
- EK1255
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- CD320 (cluster of differentiation 320), also known as 8D6A or TCBLR, is a human gene. It is mapped to chromosome 19p13.2. This gene encodes the transcobalamin receptor that is expressed at the cell surface. It mediates the cellular uptake of transcobalamin bound cobalamin (vitamin B12), and the expression of CD320 enhanced B-cell proliferation and immunoglobulin secretion. Mutations in this gene are associated with methylmalonic aciduria. It has been found that antagonists of the CD320 signaling pathway may counter the growth of follicular lymphomas or other tumors that metastasize to lymphoid follicles. CD320 is also a specific receptor for uptake of TCN2-bound cobalamin.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 293 300 313 285 297 10.28 3.4% Sample 2 1266 1315 1451 1426 1364 76.51 5.6% Sample 3 4609 4247 4591 4105 4388 217.95 4.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 293 300 313 285 297 10.28 3.4% Sample 1 293 300 313 285 297 10.28 3.4% Sample 2 1266 1315 1451 1426 1364 76.51 5.6% Sample 2 1266 1315 1451 1426 1364 76.51 5.6% Sample 3 4609 4247 4591 4105 4388 217.95 4.9% Sample 3 4609 4247 4591 4105 4388 217.95 4.9%
- Synonyms
- CD320 antigen;8D6 antigen; FDC-signaling molecule 8D6; FDC-SM-8D6; Transcobalamin receptor; TCblR; CD320; CD320;8D6A; UNQ198/PRO224
- Gene Name
- CD320
- Gene ID
- 51293
- UniProt
- Q9NPF0
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: S36-Y229
- Capture Antibody
- Monoclonal antibody from mouse
- Detection Antibody
- Polyclonal antibody from goat
- Tissue Specificity
- Expressed abundantly on follicular dendritic cells (FDCs) . .
- Applications
- ELISA
- Field of Research
- Adaptive Immunity, B Cells, Cell Biology, Cell Cycle, Cell Differentiation, Developmental Biology, Immunology, Organogenesis
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum, plasma (heparin, EDTA) and urine.
- Detection Range
- 156 pg/mL - 10,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1142 1266 4609 322 1142 5029 Standard deviation 14.65 72.16 271.93 17.06 81.08 326.88 CV (%) 5% 5.7% 5.9% 5.3% 7.1% 6.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1142 1266 4609 322 1142 5029 Standard deviation 14.65 72.16 271.93 17.06 81.08 326.88 CV (%) 5% 5.7% 5.9% 5.3% 7.1% 6.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 1142 1266 4609 322 1142 5029 Mean (pg/ml) 1142 1266 4609 322 1142 5029 Standard deviation 14.65 72.16 271.93 17.06 81.08 326.88 Standard deviation 14.65 72.16 271.93 17.06 81.08 326.88 CV (%) 5% 5.7% 5.9% 5.3% 7.1% 6.5% CV (%) 5% 5.7% 5.9% 5.3% 7.1% 6.5%
- Sensitivity
- <10 pg/mL
- Function
- Germinal center-B (GC-B) cells differentiate into memory B-cells and plasma cells (PC) through interaction with T-cells and follicular dendritic cells (FDC) . CD320 augments the proliferation of PC precursors generated by IL-10. Receptor for the cellular uptake of transcobalamin bound cobalamin. .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 28991 MW
- Specificity
- Natural and recombinant human CD320
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- CD320 antigen
- Subcellular Location
- Membrane ; Single-pass type I membrane protein .
- Protein Name
- CD320 antigen
- Sample Data
- Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.081 0.147 0.196 0.294 0.461 0.749 1.292 1.991 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.081 0.147 0.196 0.294 0.461 0.749 1.292 1.991 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.081 0.147 0.196 0.294 0.461 0.749 1.292 1.991 O.D. 0.081 0.147 0.196 0.294 0.461 0.749 1.292 1.991
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
