Human IL-18R1 ELISA Kit PicoKine®

About this product

Human IL-18R1 ELISA Kit PicoKine® (96 Tests) . Quantitate Human IL18R1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK1260

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

The interleukin-18 receptor 1 (IL-18R1), also known as CDw218a (cluster of differentiation w218a) or IL18RA, is an interleukin receptor of the immunoglobulin superfamily. The protein encoded by this gene is a cytokine receptor that belongs to the interleukin 1 receptor family. This gene along with four other members of the interleukin 1 receptor family, including IL1R2, IL1R1, ILRL2 (IL-1Rrp2), and IL1RL1 (T1/ST2), form a gene cluster on chromosome 2q. This receptor specifically binds interleukin 18 (IL18), and is essential for IL18 mediated signal transduction. IFN-alpha and IL12 are reported to induce the expression of this receptor in NK and T cells.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 73 73 69 77 73 2.82 3.8% Sample 2 260 269 256 285 267 11.14 4.1% Sample 3 905 862 955 814 884 52.12 5.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 73 73 69 77 73 2.82 3.8% Sample 1 73 73 69 77 73 2.82 3.8% Sample 2 260 269 256 285 267 11.14 4.1% Sample 2 260 269 256 285 267 11.14 4.1% Sample 3 905 862 955 814 884 52.12 5.8% Sample 3 905 862 955 814 884 52.12 5.8%

Synonyms

Interleukin-18 receptor 1; IL-18R-1; IL-18R1; CD218 antigen-like family member A; CDw218a; IL1 receptor-related protein; IL-1Rrp; IL1R-rp; CD218a; IL18R1; IL1RRP

Gene Name

IL18R1

Gene ID

8809

UniProt

Q13478

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: C22-R329

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

Expressed in lung, leukocytes, spleen, liver, thymus, prostate, small intestine, colon, placenta, and heart, and is absent from brain, skeletal muscle, pancreas, and kidney. High level of expression in Hodgkin disease cell lines.

Applications

ELISA

Field of Research

Cytokines, Immunology, Innate Immunity, Interleukins, Macrophage/Inflammation

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

31.2 pg/mL - 2,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 73 260 905 69 279 830 Standard deviation 7.25 11.96 57.20 7.37 17.85 60.59 CV (%) 7.2% 4.6% 6.1% 7.5% 6.4% 7.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 73 260 905 69 279 830 Standard deviation 7.25 11.96 57.20 7.37 17.85 60.59 CV (%) 7.2% 4.6% 6.1% 7.5% 6.4% 7.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 73 260 905 69 279 830 Mean (pg/ml) 73 260 905 69 279 830 Standard deviation 7.25 11.96 57.20 7.37 17.85 60.59 Standard deviation 7.25 11.96 57.20 7.37 17.85 60.59 CV (%) 7.2% 4.6% 6.1% 7.5% 6.4% 7.3% CV (%) 7.2% 4.6% 6.1% 7.5% 6.4% 7.3%

Sensitivity

<10 pg/mL

Function

Receptor for interleukin 18 (IL-18) . Binding to the agonist leads to the activation of NF-kappa-B.

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

62304 MW

Specificity

Natural and recombinant human IL-18R1

Gene Name Synonym

Interleukin-18 receptor 1

Subcellular Location

Membrane; Single-pass type I membrane protein.

Sequence Similarities

Belongs to the interleukin-1 receptor family.

Protein Name

Interleukin-18 receptor 1

Sample Data

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.032 0.133 0.203 0.305 0.468 0.806 1.349 2.031 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.032 0.133 0.203 0.305 0.468 0.806 1.349 2.031 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.032 0.133 0.203 0.305 0.468 0.806 1.349 2.031 O.D. 0.032 0.133 0.203 0.305 0.468 0.806 1.349 2.031

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.