- Catalog number
- EK1262
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Lumican, also known as LUM, is a protein which in humans is encoded by the LUM gene. This gene is a member of the small interstitial proteoglycan gene (SIPG) family, and it is a keratan sulfate proteoglycan which presents large quantities in the corneal stroma and in interstitial collagenous matrices of the heart, aorta, skeletal muscle, skin, and intervertebral discs. Lumican is mapped to 12q21.33, it interacts with collagen and limits growth of fibrils in diameter. In the cornea, Lumican not only interacts with collagen molecules to limit fibril growth, but also plays a critical role in the regular spacing of fibrils and acquisition of corneal transparency by virtue of its keratan sulfate-containing glycosaminoglycan side chains LDC.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 202 232 199 227 215 14.64 6.8% Sample 2 1179 1091 1283 1296 1212 83.4 6.8% Sample 3 5476 4974 5363 5047 5215 209.95 4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 202 232 199 227 215 14.64 6.8% Sample 1 202 232 199 227 215 14.64 6.8% Sample 2 1179 1091 1283 1296 1212 83.4 6.8% Sample 2 1179 1091 1283 1296 1212 83.4 6.8% Sample 3 5476 4974 5363 5047 5215 209.95 4% Sample 3 5476 4974 5363 5047 5215 209.95 4%
- Synonyms
- Lumican; Keratan sulfate proteoglycan lumican; KSPG lumican; Lum; Lcn, Ldc
- Gene Name
- LUM
- Gene ID
- 81682
- UniProt
- P51886
- Reactivity
- Rat
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: Q19-N338
- Capture Antibody
- Monoclonal antibody from mouse
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Field of Research
- Cancer, Cytoskeleton/ECM, ECM Proteins, Extracellular Matrix, Invasion/Microenvironment, Signal Transduction
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, serum and plasma (heparin) .
- Detection Range
- 156 pg/mL - 10,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 202 1179 5476 186 1201 5528 Standard deviation 13.33 54.23 339.51 16.65 58.84 414.6 CV (%) 6.6% 4.6% 6.2% 8.2% 4.9% 7.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 202 1179 5476 186 1201 5528 Standard deviation 13.33 54.23 339.51 16.65 58.84 414.6 CV (%) 6.6% 4.6% 6.2% 8.2% 4.9% 7.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 202 1179 5476 186 1201 5528 Mean (pg/ml) 202 1179 5476 186 1201 5528 Standard deviation 13.33 54.23 339.51 16.65 58.84 414.6 Standard deviation 13.33 54.23 339.51 16.65 58.84 414.6 CV (%) 6.6% 4.6% 6.2% 8.2% 4.9% 7.5% CV (%) 6.6% 4.6% 6.2% 8.2% 4.9% 7.5%
- Sensitivity
- <10 pg/mL
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 38279 MW
- Specificity
- Natural and recombinant rat Lumican
- Anticoagulant
- Heparin
- Gene Name Synonym
- Lumican
- Subcellular Location
- Secreted, extracellular space, extracellular matrix .
- Sequence Similarities
- Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class II subfamily.
- Protein Name
- Lumican
- Sample Data
- Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.03 0.099 0.165 0.291 0.51 0.832 1.35 1.921 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.03 0.099 0.165 0.291 0.51 0.832 1.35 1.921 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.03 0.099 0.165 0.291 0.51 0.832 1.35 1.921 O.D. 0.03 0.099 0.165 0.291 0.51 0.832 1.35 1.921
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
