- Catalog number
- EK1275
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Galectin-4, also known as LGALS4 or GAL4, is a protein that in humans is encoded by the LGALS4 gene. It is mapped to 19q13.2. Galectins are a family of animal lectins defined by an affinity for beta-galactoside-containing saccharides and by shared sequence elements. Galectin-4 is expressed as a 38-kD protein in the human colon adenocarcinoma T84 cell line. Galectin-4 is an S-type lectin that is strongly underexpressed in colorectal cancer, and this gene plays a role in cell adhesion. It has been found that decreased Galectin-4 expression occurs early in colorectal carcinogenesis.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 60 54 60 64 59 3.57 6% Sample 2 243 258 258 260 254 6.83 2.6% Sample 3 879 923 848 978 907 48.89 5.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 60 54 60 64 59 3.57 6% Sample 1 60 54 60 64 59 3.57 6% Sample 2 243 258 258 260 254 6.83 2.6% Sample 2 243 258 258 260 254 6.83 2.6% Sample 3 879 923 848 978 907 48.89 5.3% Sample 3 879 923 848 978 907 48.89 5.3%
- Synonyms
- Galectin-4; Gal-4; Antigen NY-CO-27; L-36 lactose-binding protein; L36LBP; Lactose-binding lectin 4; LGALS4
- Gene Name
- LGALS4
- Gene ID
- 3960
- UniProt
- P56470
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: E.coli; Immunogen sequence: A2-I323
- Capture Antibody
- Monoclonal antibody from mouse
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Field of Research
- Cancer, Cell Adhesion,2339, Cytoskeleton/ECM, Energy Metabolism, Energy Transfer Pathways, Epigenetics and Nuclear Signaling, Metabolic Signaling Pathways, Metabolism, Pathways and Processes, Signal Transduction, Tags & Cell Markers, Transcription, Transcription Factors
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 31.2 pg/mL - 2,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 60 243 879 64 223 865 Standard deviation 3.24 19.19 45.7 4.28 19.4 47.57 CV (%) 5.4% 7.9% 5.2% 6.7% 8.7% 5.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 60 243 879 64 223 865 Standard deviation 3.24 19.19 45.7 4.28 19.4 47.57 CV (%) 5.4% 7.9% 5.2% 6.7% 8.7% 5.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 60 243 879 64 223 865 Mean (pg/ml) 60 243 879 64 223 865 Standard deviation 3.24 19.19 45.7 4.28 19.4 47.57 Standard deviation 3.24 19.19 45.7 4.28 19.4 47.57 CV (%) 5.4% 7.9% 5.2% 6.7% 8.7% 5.5% CV (%) 5.4% 7.9% 5.2% 6.7% 8.7% 5.5%
- Sensitivity
- <10 pg/mL
- Function
- Galectin that binds lactose and a related range of sugars. May be involved in the assembly of adherens junctions.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 35941 MW
- Specificity
- Natural and recombinant human Galectin-4
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Galectin-4
- Sequence Similarities
- Contains 2 galectin domains.
- Protein Name
- Galectin-4
- Sample Data
- Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.067 0.125 0.18 0.283 0.482 0.847 1.568 2.589 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.067 0.125 0.18 0.283 0.482 0.847 1.568 2.589 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.067 0.125 0.18 0.283 0.482 0.847 1.568 2.589 O.D. 0.067 0.125 0.18 0.283 0.482 0.847 1.568 2.589
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
