- Catalog number
- EK1284
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Cysteine-rich secretory protein 3 is a cysteine-rich secretory protein that in humans is encoded by the CRISP3 gene. CRISP3 expression is found in the secretory epithelium throughout the male genital tract, with particularly high expression in the cauda epididymis and ampulla vas deferens. It is a potential biomarker for prostate cancer. CRISP3 gene can be defined as an early-transcribed gene with a putative role in the pathophysiology of sjogren's syndrome. CRISP3 is present in exocrine secretions and in secretory granules of neutrophilic granulocytes and is believed to play a role in innate immunity.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 110 113 130 111 116 8.15 7.0% Sample 2 383 419 441 377 405 26.26 6.4% Sample 3 1477 1600 1623 1582 1570 55.90 3.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 110 113 130 111 116 8.15 7.0% Sample 1 110 113 130 111 116 8.15 7.0% Sample 2 383 419 441 377 405 26.26 6.4% Sample 2 383 419 441 377 405 26.26 6.4% Sample 3 1477 1600 1623 1582 1570 55.90 3.5% Sample 3 1477 1600 1623 1582 1570 55.90 3.5%
- Synonyms
- Cysteine-rich secretory protein 3; CRISP-3; Specific granule protein of 28 kDa; SGP28; CRISP3
- Gene Name
- CRISP3
- Gene ID
- 10321
- UniProt
- P54108
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: N21-Y245
- Capture Antibody
- Monoclonal antibody from mouse
- Detection Antibody
- Polyclonal antibody from goat
- Tissue Specificity
- Salivary gland, pancreas and prostate > epididymis, ovary, thymus and colon.
- Applications
- ELISA
- Field of Research
- Cancer, Tumor Biomarkers
- Standard
- 100 ng/Tube
- Sample Type
- Cell culture supernatants, serum, plasma (heparin, EDTA), saliva and urine.
- Detection Range
- 62.5 pg/mL - 4,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 110 383 1477 119 408 1764 Standard deviation 4.51 23.75 66.47 5.59 26.11 95.26 CV (%) 4.1% 6.2% 4.5% 4.7% 6.4% 5.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 110 383 1477 119 408 1764 Standard deviation 4.51 23.75 66.47 5.59 26.11 95.26 CV (%) 4.1% 6.2% 4.5% 4.7% 6.4% 5.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 110 383 1477 119 408 1764 Mean (pg/ml) 110 383 1477 119 408 1764 Standard deviation 4.51 23.75 66.47 5.59 26.11 95.26 Standard deviation 4.51 23.75 66.47 5.59 26.11 95.26 CV (%) 4.1% 6.2% 4.5% 4.7% 6.4% 5.4% CV (%) 4.1% 6.2% 4.5% 4.7% 6.4% 5.4%
- Sensitivity
- <50 pg/mL
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 27630 MW
- Specificity
- Natural and recombinant human CRISP3
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Cysteine-rich secretory protein 3
- Subcellular Location
- Secreted. In neutrophils, localized in specific granules.
- Sequence Similarities
- Belongs to the CRISP family.
- Protein Name
- Cysteine-rich secretory protein 3
- Sample Data
- Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.001 0.085 0.167 0.356 0.711 1.083 1.773 2.149 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.001 0.085 0.167 0.356 0.711 1.083 1.773 2.149 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.001 0.085 0.167 0.356 0.711 1.083 1.773 2.149 O.D. 0.001 0.085 0.167 0.356 0.711 1.083 1.773 2.149
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
