- Catalog number
- EK1287
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- CD6 (Cluster of Differentiation 6) is a human protein encoded by the CD6 gene. It is mapped to 11q12.2. CD6 is expressed predominantly in peripheral T cells and mature medullary thymocytes. This gene encodes a protein found on the outer membrane of T-lymphocytes as well as some other immune cells. The encoded protein contains three scavenger receptor cysteine-rich (SRCR) domains and a binding site for an activated leukocyte cell adhesion molecule. The gene product is important for continuation of T cell activation. What’s more, this gene may be associated with susceptibility to multiple sclerosis.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 133 133 120 119 126 6.75 5.3% Sample 2 525 507 540 469 510 26.52 5.2% Sample 3 1268 1267 1220 1339 1273 42.5 3.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 133 133 120 119 126 6.75 5.3% Sample 1 133 133 120 119 126 6.75 5.3% Sample 2 525 507 540 469 510 26.52 5.2% Sample 2 525 507 540 469 510 26.52 5.2% Sample 3 1268 1267 1220 1339 1273 42.5 3.3% Sample 3 1268 1267 1220 1339 1273 42.5 3.3%
- Synonyms
- T-cell differentiation antigen CD6; CD6; Cd6
- Gene Name
- CD6
- Gene ID
- 12511
- UniProt
- Q61003
- Reactivity
- Mouse
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: L18-G396
- Capture Antibody
- Monoclonal antibody from rat
- Detection Antibody
- Polyclonal antibody from goat
- Tissue Specificity
- Expressed predominantly in thymus, lymph node and spleen.
- Applications
- ELISA
- Field of Research
- Adaptive Immunity, Hematopoietic Progenitors, Immunology, Lymphoid, Stem Cells, T Cells, T Lymphocytic Lineage
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 62.5 pg/mL - 4,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 133 525 1268 129 485 1288 Standard deviation 5.58 38.9%7 92.56 6.45 37.83 114.63 CV (%) 4.2% 6.7% 7.3% 5% 7.8% 8.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 133 525 1268 129 485 1288 Standard deviation 5.58 38.9%7 92.56 6.45 37.83 114.63 CV (%) 4.2% 6.7% 7.3% 5% 7.8% 8.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 133 525 1268 129 485 1288 Mean (pg/ml) 133 525 1268 129 485 1288 Standard deviation 5.58 38.9%7 92.56 6.45 37.83 114.63 Standard deviation 5.58 38.9%7 92.56 6.45 37.83 114.63 CV (%) 4.2% 6.7% 7.3% 5% 7.8% 8.9% CV (%) 4.2% 6.7% 7.3% 5% 7.8% 8.9%
- Sensitivity
- <10 pg/mL
- Function
- Involved in cell adhesion. Binds to CD166.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 72255 MW
- Specificity
- Natural and recombinant mouse CD6
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- T-cell differentiation antigen CD6
- Subcellular Location
- Cell membrane ; Single-pass type I membrane protein .
- Protein Name
- T-cell differentiation antigen CD6
- Sample Data
- Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.045 0.117 0.179 0.292 0.519 0.949 1.738 2.354 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.045 0.117 0.179 0.292 0.519 0.949 1.738 2.354 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.045 0.117 0.179 0.292 0.519 0.949 1.738 2.354 O.D. 0.045 0.117 0.179 0.292 0.519 0.949 1.738 2.354
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
