- Catalog number
- EK0574
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- CD40 ligand (CD40L) is a type II membrane protein of 261 amino acids on activated T cells that induces B cell proliferation and immunoglobulin secretion. It has homology with tumour necrosis factor-alpha and -beta, and has important functions in B-cell activation and differentiation. Human CD40L with 5 exons, is mapped to the proximal region of the mouse X chromosome on Xq26.3-27.1, and can be detected on T cells but is absent from B cells and monocytes. Since CD40L is expressed on platelets and released from them on activation, its predictive value as a marker for clinical outcome and the therapeutic effect of inhibition of glycoprotein IIb/IIIa receptor in patients with acute coronary syndromes was investigated. The soluble CD40L may be involved in the process of restenosis and that it exerts its effect by triggering a complex group of inflammatory reactions on endothelial and mononuclear cells.CD40L plays a central role in the pathophysiology of acute coronary syndromes, and has a role in the pathogenesis of coronary artery lesions.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 202 213 180 201 199 11.93 5.9% Sample 2 698 648 727 616 672 43.04 6.4% Sample 3 1026 1040 977 1103 1036 44.95 4.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 202 213 180 201 199 11.93 5.9% Sample 1 202 213 180 201 199 11.93 5.9% Sample 2 698 648 727 616 672 43.04 6.4% Sample 2 698 648 727 616 672 43.04 6.4% Sample 3 1026 1040 977 1103 1036 44.95 4.3% Sample 3 1026 1040 977 1103 1036 44.95 4.3%
- Synonyms
- CD40 ligand; CD40-L; T-cell antigen Gp39; TNF-related activation protein; TRAP; Tumor necrosis factor ligand superfamily member 5; CD154; CD40 ligand, membrane form; CD40 ligand, soluble form; Cd40lg; Cd40l, Tnfsf5
- Gene Name
- CD40LG
- Gene ID
- 21947
- UniProt
- P27548
- Reactivity
- Mouse
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: E61-L260
- Capture Antibody
- Monoclonal antibody from rat
- Detection Antibody
- Polyclonal antibody from goat
- Tissue Specificity
- Specifically expressed on activated CD4+ T- lymphocytes.
- Applications
- ELISA
- Field of Research
- Adaptive Immunity, Atherosclerosis, Cardiovascular, Cytokines, Immunoglobulins, Immunology, Innate Immunity, Platelets, T Cells, TNF Superfamily, Vascular Inflammation
- Standard
- 4 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 62.5 pg/mL - 4,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 202 698 1026 185 716 1058 Standard deviation 13.93 39.08 79 14.06 52.26 85.69 CV (%) 6.9% 5.6% 7.7% 7.6% 7.3% 8.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 202 698 1026 185 716 1058 Standard deviation 13.93 39.08 79 14.06 52.26 85.69 CV (%) 6.9% 5.6% 7.7% 7.6% 7.3% 8.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 202 698 1026 185 716 1058 Mean (pg/ml) 202 698 1026 185 716 1058 Standard deviation 13.93 39.08 79 14.06 52.26 85.69 Standard deviation 13.93 39.08 79 14.06 52.26 85.69 CV (%) 6.9% 5.6% 7.7% 7.6% 7.3% 8.1% CV (%) 6.9% 5.6% 7.7% 7.6% 7.3% 8.1%
- Sensitivity
- <10 pg/mL
- Function
- Cytokine that binds to TNFRSF5. Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching (By similarity) . .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 29370 MW
- Specificity
- Natural and recombinant mouse CD40L
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- CD40 ligand
- Subcellular Location
- Cell membrane; Single-pass type II membrane protein.
- Protein Name
- CD40 ligand
- Sample Data
- Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.011 0.069 0.114 0.201 0.367 0.708 1.234 1.998 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.011 0.069 0.114 0.201 0.367 0.708 1.234 1.998 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.011 0.069 0.114 0.201 0.367 0.708 1.234 1.998 O.D. 0.011 0.069 0.114 0.201 0.367 0.708 1.234 1.998
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
