- Catalog number
- EK1155
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Cadherin-2 (CDH2), also known as neural cadherin (NCAD), is a protein that in humans is encoded by the CDH2 gene. It is a classical cadherin from the cadherin superfamily. This gene is mapped to 18q12.1. Cadherin-2 is expressed in the brain, skeletal and cardiac muscle. Cadherin-2 is commonly found in cancer cells and provides a mechanism for transendothelial migration. It is a calcium dependent cell-cell adhesion glycoprotein comprising five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry. At certain central nervous system synapses, presynaptic to postsynaptic adhesion is mediated at least in part by this gene product.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1737 1604 1707 1747 1698 56.64 3.3% Sample 2 5669 6007 5146 5724 5636 310.87 5.5% Sample 3 22081 20274 22811 21203 21592 950.46 4.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1737 1604 1707 1747 1698 56.64 3.3% Sample 1 1737 1604 1707 1747 1698 56.64 3.3% Sample 2 5669 6007 5146 5724 5636 310.87 5.5% Sample 2 5669 6007 5146 5724 5636 310.87 5.5% Sample 3 22081 20274 22811 21203 21592 950.46 4.4% Sample 3 22081 20274 22811 21203 21592 950.46 4.4%
- Synonyms
- Cadherin-2; Neural cadherin; N-cadherin; CD325; Cdh2
- Gene Name
- CDH2
- Gene ID
- 12558
- UniProt
- P15116
- Reactivity
- Mouse
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: D160-A724
- Capture Antibody
- Monoclonal antibody from rat
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Field of Research
- Cadherins, Cell Adhesion, Cytoskeleton/ECM, Signal Transduction
- Standard
- 50 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 0.78 ng/mL - 50 ng/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1737 5669 22081 1767 5460 20442 Standard deviation 74.69 340.14 1236.53 102.48 425.88 1512.7 CV (%) 4.3% 6% 5.6% 5.8% 7.8% 7.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1737 5669 22081 1767 5460 20442 Standard deviation 74.69 340.14 1236.53 102.48 425.88 1512.7 CV (%) 4.3% 6% 5.6% 5.8% 7.8% 7.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 1737 5669 22081 1767 5460 20442 Mean (pg/ml) 1737 5669 22081 1767 5460 20442 Standard deviation 74.69 340.14 1236.53 102.48 425.88 1512.7 Standard deviation 74.69 340.14 1236.53 102.48 425.88 1512.7 CV (%) 4.3% 6% 5.6% 5.8% 7.8% 7.4% CV (%) 4.3% 6% 5.6% 5.8% 7.8% 7.4%
- Sensitivity
- <20 pg/mL
- Function
- Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. Acts as a regulator of neural stem cells quiescence by mediating anchorage of neural stem cells to ependymocytes in the adult subependymal zone: upon cleavage by MMP24, CDH2-mediated anchorage is affected, leading to modulate neural stem cell quiescence (PubMed:24952463) . CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density. .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 99796 MW
- Specificity
- Natural and recombinant mouse Cadherin-2
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Cadherin-2
- Subcellular Location
- Cell membrane; Single-pass type I membrane protein.
- Protein Name
- Cadherin-2
- Sample Data
- Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.031 0.169 0.2 0.28 0.401 0.66 1.14 1.88 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.031 0.169 0.2 0.28 0.401 0.66 1.14 1.88 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.031 0.169 0.2 0.28 0.401 0.66 1.14 1.88 O.D. 0.031 0.169 0.2 0.28 0.401 0.66 1.14 1.88
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
