- Catalog number
- EK1281
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Receptor Activator of Nuclear Factor κ B (RANK), also known as TRANCE Receptor, is a type I membrane protein that is expressed on the surface of osteoclasts and is involved in their activation upon ligand binding. RANK is a recently described TNF receptor family member, and its ligand, RANKL, promote survival of dendritic cells and differentiation of osteoclasts. RANK contains 383 amino acids in its intracellular domain (residues 234-616), which contain three putative TRAF-binding domains (termed I, II, and III) . RANK interacts with various TRAFs through distinct motifs and activates NF-kappaB via a novel TRAF6 interaction motif, which then activates NIK, thus leading to NF-kappaB activation, whereas RANK most likely activates JNK through a TRAF2-interacting region in RANK. The standard in this kit is recombinant human RANK with the sequence of Q29-G213 aa. It is a dipolymer which compose of two chains, and the molecular weight of each is 48kda.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 209 194 185 217 201 12.49 6.2% Sample 2 514 489 548 539 522 23 4.4% Sample 3 1401 1406 1464 1575 1461 70.05 4.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 209 194 185 217 201 12.49 6.2% Sample 1 209 194 185 217 201 12.49 6.2% Sample 2 514 489 548 539 522 23 4.4% Sample 2 514 489 548 539 522 23 4.4% Sample 3 1401 1406 1464 1575 1461 70.05 4.7% Sample 3 1401 1406 1464 1575 1461 70.05 4.7%
- Synonyms
- Protein Tnfrsf11a ; Tnfrsf11a
- Gene Name
- TNFRSF11A
- Gene ID
- 498206
- UniProt
- F1M8Z6
- Reactivity
- Rat
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: Q30-P213
- Capture Antibody
- Polyclonal antibody from goat
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Field of Research
- Adapters, Atherosclerosis, Cardiovascular, Cytokines, Cytoskeleton/ECM, Extracellular Matrix, Growth Factors/Hormones, Immunology, Innate Immunity, Signal Transduction, Structures, TNF Superfamily, Transmembrane, Vascular Inflammation
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants and serum.
- Detection Range
- 62.5 pg/mL - 4,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 209 514 1401 226 542 1518 Standard deviation 13.58 34.95 88.16 18.3 42.81 97.15 CV (%) 6.5% 6.8% 7.9% 8.1% 7.9% 6.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 209 514 1401 226 542 1518 Standard deviation 13.58 34.95 88.16 18.3 42.81 97.15 CV (%) 6.5% 6.8% 7.9% 8.1% 7.9% 6.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 209 514 1401 226 542 1518 Mean (pg/ml) 209 514 1401 226 542 1518 Standard deviation 13.58 34.95 88.16 18.3 42.81 97.15 Standard deviation 13.58 34.95 88.16 18.3 42.81 97.15 CV (%) 6.5% 6.8% 7.9% 8.1% 7.9% 6.4% CV (%) 6.5% 6.8% 7.9% 8.1% 7.9% 6.4%
- Sensitivity
- <10 pg/mL
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 67607 MW
- Specificity
- Natural and recombinant rat RANK
- Gene Name Synonym
- Tumor necrosis factor receptor superfamily, member 11a, NFKB activator
- Protein Name
- Tumor necrosis factor receptor superfamily member 11A
- Sample Data
- Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.067 0.125 0.175 0.25 0.45 0.848 1.489 2.053 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.067 0.125 0.175 0.25 0.45 0.848 1.489 2.053 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.067 0.125 0.175 0.25 0.45 0.848 1.489 2.053 O.D. 0.067 0.125 0.175 0.25 0.45 0.848 1.489 2.053
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
