Rat Angiopoietin-1 ELISA Kit PicoKine®

About this product

Rat Angiopoietin-1 ELISA Kit PicoKine® (96 Tests) . Quantitate Rat Angpt1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Specifications

Catalog number

EK1295

Size / count

96 Well With Removable Strips

Dry ice

No

Hazardous

No

Discontinued

No

Background

Angiopoietin 1, also called ANG1 is a type of angiopoietin and is encoded by the gene ANGPT1. Angiopoietins are proteins with important roles in vascular development and angiogenesis. All angiopoietins bind with similar affinity to an endothelial cell-specific tyrosine-protein kinase receptor. This gene was mapped to 8q23.1. The protein encoded by this gene is a secreted glycoprotein that activates the receptor by inducing its tyrosine phosphorylation. It plays a critical role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme and inhibits endothelial permeability. The protein also contributes to blood vessel maturation and stability, and may be involved in early development of the heart.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 311 293 312 282 299 12.61 4.2% Sample 2 1285 1071 1253 1292 1225 90.26 7.3% Sample 3 4930 5240 5287 4371 4957 365.07 7.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 311 293 312 282 299 12.61 4.2% Sample 1 311 293 312 282 299 12.61 4.2% Sample 2 1285 1071 1253 1292 1225 90.26 7.3% Sample 2 1285 1071 1253 1292 1225 90.26 7.3% Sample 3 4930 5240 5287 4371 4957 365.07 7.3% Sample 3 4930 5240 5287 4371 4957 365.07 7.3%

Synonyms

Angiopoietin-1; ANG-1; Angpt1

Gene Name

ANGPT1

Gene ID

89807

UniProt

O35460

Reactivity

Rat

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: H16-F498

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Field of Research

Growth Factors

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 311 1285 4930 287 1289 5041 Standard deviation 18.97 60.39 295.8 18.65 70.89 317.58 CV (%) 6.1% 4.7% 6% 6.5% 5.5% 6.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 311 1285 4930 287 1289 5041 Standard deviation 18.97 60.39 295.8 18.65 70.89 317.58 CV (%) 6.1% 4.7% 6% 6.5% 5.5% 6.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 311 1285 4930 287 1289 5041 Mean (pg/ml) 311 1285 4930 287 1289 5041 Standard deviation 18.97 60.39 295.8 18.65 70.89 317.58 Standard deviation 18.97 60.39 295.8 18.65 70.89 317.58 CV (%) 6.1% 4.7% 6% 6.5% 5.5% 6.3% CV (%) 6.1% 4.7% 6% 6.5% 5.5% 6.3%

Sensitivity

<10 pg/mL

Function

Binds and activates TIE2 receptor by inducing its tyrosine phosphorylation. Implicated in endothelial developmental processes later and distinct from that of VEGF. Appears to play a crucial role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme. Mediates blood vessel maturation/stability. It may play an important role in the heart early development.

Components

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

57461 MW

Specificity

Natural and recombinant rat Angiopoietin-1

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Angiopoietin-1

Subcellular Location

Secreted.

Protein Name

Angiopoietin-1

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.04 0.109 0.198 0.311 0.685 1.131 1.69 2.289 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.04 0.109 0.198 0.311 0.685 1.131 1.69 2.289 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.04 0.109 0.198 0.311 0.685 1.131 1.69 2.289 O.D. 0.04 0.109 0.198 0.311 0.685 1.131 1.69 2.289

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.