- Catalog number
- EK1298
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Insulin-like growth factor-binding protein 5 is a protein that in humans is encoded by the IGFBP5 gene. The IGFBP5 gene was mapped to chromosome 2q33-q34. The expression of IGFBP5 by stable transfection and adenovirus-mediated infection was inhibitory to growth in 2 human breast cancer cell lines. Stable expression of IGFBP5 in the breast cancer cell lines also inhibited the formation and growth of tumors following injection in athymic mice. IGFBP5 is believed a growth inhibitor and proapoptotic agent in breast cancer cells.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 315 305 301 291 303 8.6 2.8% Sample 2 1184 1112 1004 1119 1104 64.58 5.8% Sample 3 5793 5090 4974 4943 5200 346.72 6.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 315 305 301 291 303 8.6 2.8% Sample 1 315 305 301 291 303 8.6 2.8% Sample 2 1184 1112 1004 1119 1104 64.58 5.8% Sample 2 1184 1112 1004 1119 1104 64.58 5.8% Sample 3 5793 5090 4974 4943 5200 346.72 6.6% Sample 3 5793 5090 4974 4943 5200 346.72 6.6%
- Synonyms
- Insulin-like growth factor-binding protein 5; IBP-5; IGF-binding protein 5; IGFBP-5; Igfbp5; Igfbp-5
- Gene Name
- IGFBP5
- Gene ID
- 25285
- UniProt
- P24594
- Reactivity
- Rat
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: L20-E271
- Capture Antibody
- Monoclonal antibody from mouse
- Detection Antibody
- Polyclonal antibody from goat
- Tissue Specificity
- Mostly in kidney.
- Applications
- ELISA
- Field of Research
- Growth Factors/Hormones, Lipid Signaling, Signal Transduction, Signaling Pathway
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 156 pg/mL - 10,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 315 1184 5793 328 1134 5772 Standard deviation 24.25 73.4 341.78 26.24 85.05 369.4 CV (%) 7.7% 6.2% 5.9% 8% 7.5% 6.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 315 1184 5793 328 1134 5772 Standard deviation 24.25 73.4 341.78 26.24 85.05 369.4 CV (%) 7.7% 6.2% 5.9% 8% 7.5% 6.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 315 1184 5793 328 1134 5772 Mean (pg/ml) 315 1184 5793 328 1134 5772 Standard deviation 24.25 73.4 341.78 26.24 85.05 369.4 Standard deviation 24.25 73.4 341.78 26.24 85.05 369.4 CV (%) 7.7% 6.2% 5.9% 8% 7.5% 6.4% CV (%) 7.7% 6.2% 5.9% 8% 7.5% 6.4%
- Sensitivity
- <10 pg/mL
- Function
- IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 30298 MW
- Specificity
- Natural and recombinant rat IGFBP5
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Insulin-like growth factor-binding protein 5
- Subcellular Location
- Secreted.
- Sequence Similarities
- Contains 1 IGFBP N-terminal domain.
- Protein Name
- Insulin-like growth factor-binding protein 5
- Sample Data
- Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.042 0.065 0.099 0.145 0.263 0.522 1.135 1.906 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.042 0.065 0.099 0.145 0.263 0.522 1.135 1.906 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.042 0.065 0.099 0.145 0.263 0.522 1.135 1.906 O.D. 0.042 0.065 0.099 0.145 0.263 0.522 1.135 1.906
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
