- Catalog number
- EK1302
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- TIMP-2 gen is encoded by 5 exons spanning 83 kb of genomic DNA. TIMP-2 is 83 kilobase pairs (kb) long with exon-intron splicing sites located in preserved positions among the three members of the TIMP family. The gene for tissue inhibitor of metalloproteinases-2 is localized on human chromosome arm 17q25. TIMP-2 abrogates angiogenic factor-induced endothelial cell proliferation in vitro and angiogenesis in vivo independent of MMP inhibition. The standard product used in this kit is recombinant human TIMP-2 with the molecular mass of 22Kda and 194 amino acid.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 240 251 238 213 235 13.9 5.9% Sample 2 1342 1286 1409 1375 1353 45.35 3.3% Sample 3 4533 4407 4200 4479 4404 126.38 2.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 240 251 238 213 235 13.9 5.9% Sample 1 240 251 238 213 235 13.9 5.9% Sample 2 1342 1286 1409 1375 1353 45.35 3.3% Sample 2 1342 1286 1409 1375 1353 45.35 3.3% Sample 3 4533 4407 4200 4479 4404 126.38 2.8% Sample 3 4533 4407 4200 4479 4404 126.38 2.8%
- Synonyms
- Metalloproteinase inhibitor 2; Tissue inhibitor of metalloproteinases 2; TIMP-2; Timp2; Timp-2
- Gene Name
- TIMP2
- Gene ID
- 29543
- UniProt
- P30121
- Reactivity
- Rat
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: C27-P220
- Capture Antibody
- Polyclonal antibody from goat
- Detection Antibody
- Polyclonal antibody from goat
- Applications
- ELISA
- Field of Research
- Angiogenesis, Atherosclerosis, Cancer, Cardiovascular, Cell Biology, Cytoskeleton/ECM, ECM Enzymes, Extracellular Matrix, Invasion/Microenvironment, Protease Inhibitors, Proteolysis/Ubiquitin, Signal Transduction
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 156 pg/mL - 10,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 240 1342 4533 227 1463 4769 Standard deviation 10.56 68.44 339.97 10.21 95.09 443.51 CV (%) 4.4% 9.3% 7.5% 4.5% 6.5% 9.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 240 1342 4533 227 1463 4769 Standard deviation 10.56 68.44 339.97 10.21 95.09 443.51 CV (%) 4.4% 9.3% 7.5% 4.5% 6.5% 9.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 240 1342 4533 227 1463 4769 Mean (pg/ml) 240 1342 4533 227 1463 4769 Standard deviation 10.56 68.44 339.97 10.21 95.09 443.51 Standard deviation 10.56 68.44 339.97 10.21 95.09 443.51 CV (%) 4.4% 9.3% 7.5% 4.5% 6.5% 9.3% CV (%) 4.4% 9.3% 7.5% 4.5% 6.5% 9.3%
- Sensitivity
- <10 pg/mL
- Function
- Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor.
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 24356 MW
- Specificity
- Natural and recombinant rat TIMP-2
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Metalloproteinase inhibitor 2
- Subcellular Location
- Secreted.
- Sequence Similarities
- Belongs to the protease inhibitor I35 (TIMP) family.
- Protein Name
- Metalloproteinase inhibitor 2
- Sample Data
- Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.024 0.141 0.248 0.431 0.857 1.39 1.932 2.243 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.024 0.141 0.248 0.431 0.857 1.39 1.932 2.243 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.024 0.141 0.248 0.431 0.857 1.39 1.932 2.243 O.D. 0.024 0.141 0.248 0.431 0.857 1.39 1.932 2.243
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
