- Catalog number
- EK1304
- Size / count
- 96 Well With Removable Strips
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- SIGLEC-1, also known as Sialoadhesin or CD169, is a cell adhesion molecule found on the surface of certain cells of the immune system called macrophages. This gene is mapped to 20p13. It belongs to the immunoglobulin superfamily (IgSF) . Since sialoadhesin binds sialic acids with its N-terminal IgV-domain, it is also a member of the SIGLEC family. The localization and expression of SIGLEC-1 in humans has altered coincident with the evolutionary loss of Neu5Gc. SIGLEC-1-positive macrophages have a dual physiologic function. They act as innate 'flypaper' by preventing the systemic spread of lymph-borne pathogens and as critical gatekeepers at the lymph-tissue interface that facilitate the recognition of particulate antigens by B cells and initiate humoral immune responses.
- Reproducibility
- Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 278 276 287 263 276 8.57 3.1% Sample 2 1821 2107 2081 1805 1953 140.91 7.2% Sample 3 4073 4478 4741 4142 4358 268.77 6.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 278 276 287 263 276 8.57 3.1% Sample 1 278 276 287 263 276 8.57 3.1% Sample 2 1821 2107 2081 1805 1953 140.91 7.2% Sample 2 1821 2107 2081 1805 1953 140.91 7.2% Sample 3 4073 4478 4741 4142 4358 268.77 6.1% Sample 3 4073 4478 4741 4142 4358 268.77 6.1%
- Synonyms
- Sialoadhesin; Sialic acid-binding Ig-like lectin 1; Siglec-1; CD169; SIGLEC1; SN
- Gene Name
- SIGLEC1
- Gene ID
- 6614
- UniProt
- Q9BZZ2
- Reactivity
- Human
- Cross Reactivity
- There is no detectable cross-reactivity with other relevant proteins.
- Immunogen
- Expression system for standard: NS0; Immunogen sequence: S20-Q1641
- Capture Antibody
- Monoclonal antibody from mouse
- Detection Antibody
- Polyclonal antibody from goat
- Tissue Specificity
- Expressed by macrophages in various tissues. High levels are found in spleen, lymph node, perivascular macrophages in brain and lower levels in bone marrow, liver Kupffer cells and lamina propria of colon and lung. Also expressed by inflammatory macrophages in rheumatoid arthritis.
- Applications
- ELISA
- Field of Research
- Cell Type Markers, Immunology, Innate Immunity, Macrophage/Inflammation, Tags & Cell Markers
- Standard
- 10 ng/Tube
- Sample Type
- Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .
- Detection Range
- 156 pg/mL - 10,000 pg/mL
- Precision
- Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 278 1821 4073 286 1650 3922 Standard deviation 20.01 138.39 240.3 22.59 148.5 262.77 CV (%) 7.2% 7.6% 5.9% 7.9% 9% 6.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 278 1821 4073 286 1650 3922 Standard deviation 20.01 138.39 240.3 22.59 148.5 262.77 CV (%) 7.2% 7.6% 5.9% 7.9% 9% 6.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 278 1821 4073 286 1650 3922 Mean (pg/ml) 278 1821 4073 286 1650 3922 Standard deviation 20.01 138.39 240.3 22.59 148.5 262.77 Standard deviation 20.01 138.39 240.3 22.59 148.5 262.77 CV (%) 7.2% 7.6% 5.9% 7.9% 9% 6.7% CV (%) 7.2% 7.6% 5.9% 7.9% 9% 6.7%
- Sensitivity
- <10 pg/mL
- Function
- Acts as an endocytic receptor mediating clathrin dependent endocytosis. Macrophage-restricted adhesion molecule that mediates sialic-acid dependent binding to lymphocytes, including granulocytes, monocytes, natural killer cells, B-cells and CD8 T-cells. Preferentially binds to alpha-2,3-linked sialic acid (By similarity) . Binds to SPN/CD43 on T-cells (By similarity) . May play a role in hemopoiesis. .
- Components
- Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4
- Storage Conditions
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
- Calculated Molecular Weight
- 182624 MW
- Specificity
- Natural and recombinant human CD169
- Anticoagulant
- Heparin or EDTA
- Gene Name Synonym
- Sialoadhesin
- Subcellular Location
- Isoform 1: Cell membrane; Single-pass type I membrane protein.
- Sequence Similarities
- Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
- Protein Name
- Sialoadhesin
- Sample Data
- Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.019 0.095 0.153 0.285 0.507 0.96 1.669 2.326 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.019 0.095 0.153 0.285 0.507 0.96 1.669 2.326 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.019 0.095 0.153 0.285 0.507 0.96 1.669 2.326 O.D. 0.019 0.095 0.153 0.285 0.507 0.96 1.669 2.326
- Material Required But Not Provided
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
